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Association of the interleukin-2 polymorphisms with interleukin-2 serum levels and risk of nasopharyngeal carcinoma.
- Source :
-
DNA and cell biology [DNA Cell Biol] 2010 Jul; Vol. 29 (7), pp. 363-8. - Publication Year :
- 2010
-
Abstract
- Nasopharyngeal carcinoma (NPC) is one of the most common cancers in southern China. In addition to environmental factors such as Epstein-Barr virus infection and chemical carcinogen exposure, genetic susceptibility has been reported to play a key role in the development of this disease. Interleukin-2 (IL-2) is an immunoregulatory cytokine produced by T cells and plays an important role in antitumor immunity. Variations in the DNA sequence of the IL-2 gene may lead to altered cytokine production and/or activity, and thus modulate an individual's susceptibility to NPC. To test this hypothesis, we investigated whether IL-2 gene polymorphisms and its serum levels are associated with NPC in a Chinese population. We analyzed single-nucleotide polymorphisms of IL-2 gene -330 T/G and +114 T/G in 180 patients with NPC and 200 age- and sex-matched controls, using polymerase chain reaction-restriction fragment length polymorphism and DNA sequencing methods, and serum IL-2 levels were measured by enzyme-linked immunosorbent assay. Serum IL-2 levels were decreased in patients with NPC compared with controls (p < 0.01). There were significant differences in the genotype and allele frequencies of IL-2 gene -330 T/G polymorphism between the group of patients with NPC and the control group (p < 0.05). Moreover, genotypes carrying the IL-2 -330 G variant allele were associated with decreased serum IL-2 levels compared with the homozygous wild-type genotype in patients with NPC. Carrying the IL-2 -330 G variant allele was associated with a decreased ability to produce IL-2, which may contribute to NPC susceptibility.
- Subjects :
- Alleles
Asian People genetics
Base Sequence
China epidemiology
Enzyme-Linked Immunosorbent Assay
Gene Frequency
Genetic Predisposition to Disease
Genotype
Herpesvirus 4, Human genetics
Homozygote
Humans
Nasopharyngeal Neoplasms epidemiology
Nasopharyngeal Neoplasms pathology
Polymerase Chain Reaction
Polymorphism, Restriction Fragment Length
Polymorphism, Single Nucleotide
Risk Factors
Interleukin-2 genetics
Nasopharyngeal Neoplasms genetics
Polymorphism, Genetic
Subjects
Details
- Language :
- English
- ISSN :
- 1557-7430
- Volume :
- 29
- Issue :
- 7
- Database :
- MEDLINE
- Journal :
- DNA and cell biology
- Publication Type :
- Academic Journal
- Accession number :
- 20438365
- Full Text :
- https://doi.org/10.1089/dna.2010.1019