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EIF2alpha and caspase-12 activation are involved in oxygen-glucose-serum deprivation/restoration-induced apoptosis of spinal cord astrocytes.
- Source :
-
Neuroscience letters [Neurosci Lett] 2010 Jun 30; Vol. 478 (1), pp. 32-6. Date of Electronic Publication: 2010 May 02. - Publication Year :
- 2010
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Abstract
- Astrocytes play an important role in protecting neurons during ischemia and reperfusion in the central nervous system. Although many studies have shown that oxygen-glucose deprivation (OGD) can induce astrocyte apoptosis, the role of PERK/eIF2 alpha/ATF4 integrated stress response (ISR) in astrocyte apoptosis mediated by oxygen-glucose-serum deprivation (OGSD)/restoration remains uncertain. Astrocytes were subjected to a combination of oxygen, glucose, and serum deprivation for 8h followed by restoration. Hoechst 33342 staining was performed to quantify apoptotic astrocytes and cell viability was assessed with Cell Counting Kit-8 (CCK8). Immunocytochemical analysis and Western blotting for some related molecules, including pancreatic ER stress kinase (PERK), p-PERK, eukaryotic initiation factor 2 alpha (eIF2 alpha), p-eIF2 alpha, activating transcription factor 4 (ATF4), caspase-12, were examined. Caspase activation and apoptosis were detected in neonatal rat astrocytes from spinal cord subjected to OGSD/restoration. We also observed an increase in cytoplasmic staining of p-eIF2 alpha in astrocytes (8h OGSD/15 min restoration) compared with that of non-treated cells. In addition, we found the sequential activation of PERK, eIF2 alpha, and ATF4 during OGSD/restoration by Western blotting. These results indicate that both the PERK/eIF2 alpha/ATF4 ISR and activation of caspase-12 may be involved in apoptosis of spinal cord astrocytes induced by OGSD/restoration.<br /> ((c) 2010 Elsevier Ireland Ltd. All rights reserved.)
- Subjects :
- Activating Transcription Factor 4 physiology
Animals
Animals, Newborn
Astrocytes metabolism
Cell Survival
Cells, Cultured
Culture Media, Serum-Free
Enzyme Activation
Rats
Rats, Sprague-Dawley
Spinal Cord metabolism
eIF-2 Kinase physiology
Apoptosis
Astrocytes cytology
Caspase 12 physiology
Eukaryotic Initiation Factor-2 physiology
Glucose deficiency
Oxygen metabolism
Spinal Cord cytology
Subjects
Details
- Language :
- English
- ISSN :
- 1872-7972
- Volume :
- 478
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Neuroscience letters
- Publication Type :
- Academic Journal
- Accession number :
- 20444431
- Full Text :
- https://doi.org/10.1016/j.neulet.2010.04.062