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Satellite glia not DRG neurons constitutively activate EGFR but EGFR inactivation is not correlated with axon regeneration.
- Source :
-
Neurobiology of disease [Neurobiol Dis] 2010 Sep; Vol. 39 (3), pp. 292-300. Date of Electronic Publication: 2010 May 06. - Publication Year :
- 2010
-
Abstract
- To test the possibility that phosphorylated epidermal growth factor receptor (pEGFR) mediates axon growth inhibition, we determined if pEGFR levels were raised in dorsal root ganglia (DRG) after non-regenerating dorsal column (DC) lesions and suppressed in regenerating sciatic nerve (SN) and preconditioning (P) SN+DC lesioned DRG. Levels of EGFR mRNA and protein in DRG were unchanged between control and all injury models. Satellite glia and not DRG neurons (DRGN) constitutively contained pEGFR and, only in PSN+DC rats, were levels significantly reduced in these cells. In vitro, siRNA mediated knockdown of EGFR (siEGFR) mRNA and protein was associated with suppressed RhoA activation, but fibroblast growth factor-2 (FGF2) was a mandatory requirement for DRGN neuritogenesis after addition of inhibitory concentrations of CNS myelin. Thus, EGFR activation in satellite glia was not consistently correlated with DRGN axogenesis and siEGFR reduction of pEGFR with attenuated Rho-GTP signalling did not promote DRGN disinhibited neurite outgrowth without exogenous FGF2 stimulation. Together, these data argue against a direct intra-axonal involvement of pEGFR in axon regeneration.
- Subjects :
- Activating Transcription Factor 3 metabolism
Analysis of Variance
Animals
Blotting, Western
Cells, Cultured
GAP-43 Protein metabolism
Galanin metabolism
Immunohistochemistry
Male
Nerve Crush
Neurons cytology
Neuropeptide Y metabolism
Oligonucleotide Array Sequence Analysis
Phosphorylation
Rats
Rats, Sprague-Dawley
Reverse Transcriptase Polymerase Chain Reaction
Axons metabolism
ErbB Receptors metabolism
Ganglia, Spinal metabolism
Nerve Regeneration physiology
Neuroglia metabolism
Neurons metabolism
Sciatic Nerve physiology
Subjects
Details
- Language :
- English
- ISSN :
- 1095-953X
- Volume :
- 39
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Neurobiology of disease
- Publication Type :
- Academic Journal
- Accession number :
- 20451608
- Full Text :
- https://doi.org/10.1016/j.nbd.2010.04.013