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Deglycosylation of HIV-1 AE Gp140 enhances the capacity to elicit neutralizing antibodies against the heterologous HIV-1 clade.
- Source :
-
AIDS research and human retroviruses [AIDS Res Hum Retroviruses] 2010 May; Vol. 26 (5), pp. 569-75. - Publication Year :
- 2010
-
Abstract
- The aim of this study was to test whether deglycosylation of an HIV-1 AE recombinant-derived gp140 could enhance the induction of neutralizing antibodies. N-to-Q mutations were introduced in the V1/V2 (m157/161) or V4 (m382/388) loops by using overlapping PCR. BALB/c mice were inoculated with different DNA vaccines at weeks 0, 2, 4, and 7. The Elispot assay was used to quantify Env-specific T-cell immunity, and the TZM-bl cell-based in vitro neutralizing assay with primary isolates was used to assess humoral immune responses. Our data showed that two mutant DNA vaccines, designated m157/161 and m382/388, mounted total T-cell responses that were at levels similar those of the unmutated vaccine. Although the levels of binding antibodies elicited by the two mutants were significantly lower than the levels elicited by the unmutated vaccine, cross-reactive neutralizing antibodies were observed only in the sera that received the mutant DNA vaccines. These data demonstrate that deglycosylation of HIV-1 Env could enhance the capacity to elicit cross-reactive neutralizing antibodies.
- Subjects :
- Animals
Cell Line
Cross Reactions immunology
Glycosylation
HIV Antibodies blood
HIV Infections immunology
HIV-1 classification
Humans
Immunization
Mice
Mice, Inbred BALB C
Models, Molecular
Mutation
Neutralization Tests
Polymerase Chain Reaction methods
Recombinant Proteins genetics
Recombinant Proteins immunology
Recombinant Proteins metabolism
T-Lymphocytes immunology
AIDS Vaccines administration & dosage
AIDS Vaccines immunology
Antibodies, Neutralizing blood
HIV Infections prevention & control
HIV-1 immunology
Vaccines, DNA administration & dosage
Vaccines, DNA immunology
env Gene Products, Human Immunodeficiency Virus chemistry
env Gene Products, Human Immunodeficiency Virus genetics
env Gene Products, Human Immunodeficiency Virus immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1931-8405
- Volume :
- 26
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- AIDS research and human retroviruses
- Publication Type :
- Academic Journal
- Accession number :
- 20455767
- Full Text :
- https://doi.org/10.1089/aid.2009.0228