Composition and immunotoxicity activity of essential oils from Lindera obtusiloba Blume against Aedes aegypti L.

The leaves of Lindera obtusiloba Blume var. obtusiloba were extracted and the major essential oil composition and immunotoxicity effects were studied. The analyses were conducted by gas chromatography and mass spectroscopy (GC-MS) revealed that the essential oils of L. obtusiloba. The L. obtusiloba essential oil yield was 4.23%, and GC/MS analysis revealed that its major constituents were α-copaene (31.42%), β-caryophyllene (32.11%), α-humulene (4.12%), β-farnesene (4.15%), α- cadinene (3.21%) and Nerolidol (6.84%). The essential oil had a significant toxic effect against early fourth-stage larvae of Aedes aegypti L with an LC(50) value of 24.32 ppm and an LC(90) value of 36.42 ppm.