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The Asia Oceania Human Proteome Organisation Membrane Proteomics Initiative. Preparation and characterisation of the carbonate-washed membrane standard.

Authors :
Peng L
Kapp EA
Fenyƶ D
Kwon MS
Jiang P
Wu S
Jiang Y
Aguilar MI
Ahmed N
Baker MS
Cai Z
Chen YJ
Van Chi P
Chung MC
He F
Len AC
Liao PC
Nakamura K
Ngai SM
Paik YK
Pan TL
Poon TC
Salekdeh GH
Simpson RJ
Sirdeshmukh R
Srisomsap C
Svasti J
Tyan YC
Dreyer FS
McLauchlan D
Rawson P
Jordan TW
Source :
Proteomics [Proteomics] 2010 Nov; Vol. 10 (22), pp. 4142-8.
Publication Year :
2010

Abstract

The Asia Oceania Human Proteome Organisation (AOHUPO) has embarked on a Membrane Proteomics Initiative with goals of systematic comparison of strategies for analysis of membrane proteomes and discovery of membrane proteins. This multilaboratory project is based on the analysis of a subcellular fraction from mouse liver that contains endoplasmic reticulum and other organelles. In this study, we present the strategy used for the preparation and initial characterization of the membrane sample, including validation that the carbonate-washing step enriches for integral and lipid-anchored membrane proteins. Analysis of 17 independent data sets from five types of proteomic workflows is in progress.<br /> (Copyright © 2010 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.)

Details

Language :
English
ISSN :
1615-9861
Volume :
10
Issue :
22
Database :
MEDLINE
Journal :
Proteomics
Publication Type :
Academic Journal
Accession number :
20486120
Full Text :
https://doi.org/10.1002/pmic.201000126