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Negative regulation of tumor suppressor p53 by microRNA miR-504.
- Source :
-
Molecular cell [Mol Cell] 2010 Jun 11; Vol. 38 (5), pp. 689-99. - Publication Year :
- 2010
-
Abstract
- Tumor suppressor p53 plays a central role in tumor prevention. p53 protein levels and activity are under a tight and complex regulation in cells to maintain the proper function of p53. MicroRNAs play a key role in the regulation of gene expression. Here we report the regulation of p53 through miR-504. miR-504 acts as a negative regulator of human p53 through its direct binding to two sites in the p53 3' untranslated region. Overexpression of miR-504 decreases p53 protein levels and functions in cells, including p53 transcriptional activity, p53-mediated apoptosis, and cell-cycle arrest in response to stress, and furthermore promotes tumorigenecity of cells in vivo. These results demonstrate the direct negative regulation of p53 by miR-504 as a mechanism for p53 regulation in cells, which highlights the importance of microRNAs in tumorigenesis.<br /> (Copyright (c) 2010 Elsevier Inc. All rights reserved.)
- Subjects :
- 3' Untranslated Regions
Animals
Apoptosis physiology
Binding Sites
Cell Cycle physiology
Cell Line, Tumor
Cell Transplantation
Female
Gene Knockdown Techniques
Genes, Reporter
Humans
Mice
Mice, Inbred BALB C
Mice, Nude
MicroRNAs genetics
Stress, Physiological
Transcription, Genetic
Tumor Suppressor Protein p53 genetics
MicroRNAs metabolism
Tumor Suppressor Protein p53 metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1097-4164
- Volume :
- 38
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Molecular cell
- Publication Type :
- Academic Journal
- Accession number :
- 20542001
- Full Text :
- https://doi.org/10.1016/j.molcel.2010.05.027