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Leishmania species: detection and identification by nested PCR assay from skin samples of rodent reservoirs.
- Source :
-
Experimental parasitology [Exp Parasitol] 2010 Dec; Vol. 126 (4), pp. 552-6. Date of Electronic Publication: 2010 Jun 08. - Publication Year :
- 2010
-
Abstract
- Many rodent species act as reservoir hosts of zoonotic cutaneous leishmaniasis in endemic areas. In the present study a simple and reliable assay based on nested PCR was developed for the detection and identification of Leishmania parasites from rodent skin samples. We designed Leishmania-specific primers that successfully amplified ITS regions of Leishmania major, Leishmania gerbilli and Leishmania turanica using nested PCR. Out of 95 field collected Rhombomys opimus, 21 were positive by microscopic examination and 48 by nested PCR. The percentage of gerbils infected with L. major, L. gerbilli and L. turanica was 3.2%, 1.1% and 27.4%, respectively. In 15.8% of the rodents, we found mixed natural infections by L. major and L. turanica, 1.1% by L. major and L. gerbilli, and 2.1% by the three species. We concluded that this method is simple and reliable for detecting and identifying Leishmania species circulating in rodent populations.
- Subjects :
- Animals
DNA Primers chemistry
DNA, Protozoan chemistry
DNA, Protozoan isolation & purification
Electrophoresis, Agar Gel veterinary
Iran epidemiology
Leishmania classification
Leishmania genetics
Leishmaniasis, Cutaneous epidemiology
Leishmaniasis, Cutaneous parasitology
Polymerase Chain Reaction veterinary
Rodent Diseases epidemiology
Disease Reservoirs parasitology
Gerbillinae parasitology
Leishmania isolation & purification
Leishmaniasis, Cutaneous veterinary
Rodent Diseases parasitology
Skin parasitology
Subjects
Details
- Language :
- English
- ISSN :
- 1090-2449
- Volume :
- 126
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Experimental parasitology
- Publication Type :
- Academic Journal
- Accession number :
- 20566364
- Full Text :
- https://doi.org/10.1016/j.exppara.2010.06.003