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Prenatal diagnosis for a novel splice mutation of PHEX gene in a large Han Chinese family affected with X-linked hypophosphatemic rickets.
- Source :
-
Genetic testing and molecular biomarkers [Genet Test Mol Biomarkers] 2010 Jun; Vol. 14 (3), pp. 385-91. - Publication Year :
- 2010
-
Abstract
- Background: X-linked hypophosphatemia (XLH) is the most common form of heritable rickets characterized by X-linked dominant inheritance, renal phosphate wasting, hypophosphatemia, and defective bone mineralization. Inactivating mutations of the PHEX gene located at Xp22.1 have been linked with this disease. Ethnic distribution of such mutations seems widespread but only a few mutations in the Chinese population have been reported to date.<br />Materials and Methods: We report on a large Han Chinese family affected with XLH rickets, which included 13 patients from four generations. Polymerase chain reaction and direct sequencing were performed for all exons and intron-exon boundaries of the PHEX gene. The effect of nucleotide changes was analyzed using bioinformatic software. Prenatal diagnosis was performed on umbilical cord blood at the 20th gestational week.<br />Results: A novel G-->A splice mutation in intron 7 (c.849+1G>A) was identified in all patients from the family. As confirmed by reverse-transcription (RT)-polymerase chain reaction (PCR), the mutation has rendered loss of a normal splice donor site (c.849+1G) while activating a cryptic one at c.849+519G, which resulted in addition of 518 nucleotides to the mature RNA. Prenatal diagnosis had excluded the fetus for carrying the same mutation. A healthy boy was born later.<br />Conclusions: A novel splice mutation c.849+1G>A in the PHEX gene is responsible for XLH in the studied family. Further studies may enhance our understanding of the role of this mutation in the pathogenesis of XLH.
- Subjects :
- China ethnology
Computational Biology
Exons genetics
Familial Hypophosphatemic Rickets genetics
Family
Female
Humans
Introns genetics
Male
Pedigree
Polymerase Chain Reaction
Pregnancy
Sequence Analysis, DNA
Alternative Splicing
Familial Hypophosphatemic Rickets diagnosis
Genetic Diseases, X-Linked
Mutation
PHEX Phosphate Regulating Neutral Endopeptidase genetics
Prenatal Diagnosis
Subjects
Details
- Language :
- English
- ISSN :
- 1945-0257
- Volume :
- 14
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Genetic testing and molecular biomarkers
- Publication Type :
- Academic Journal
- Accession number :
- 20578943
- Full Text :
- https://doi.org/10.1089/gtmb.2009.0175