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Nardostachys jatamansi extract protects against cytokine-induced beta-cell damage and streptozotocin-induced diabetes.
- Source :
-
World journal of gastroenterology [World J Gastroenterol] 2010 Jul 14; Vol. 16 (26), pp. 3249-57. - Publication Year :
- 2010
-
Abstract
- Aim: To investigate the anti-diabetogenic mechanism of Nardostachys jatamansi extract (NJE).<br />Methods: Mice were injected with streptozotocin via a tail vein to induce diabetes. Rat insulinoma RINm5F cells and isolated rat islets were treated with interleukin-1beta and interferon-gamma to induce cytotoxicity.<br />Results: Treatment of mice with streptozotocin resulted in hyperglycemia and hypoinsulinemia, which was confirmed by immunohistochemical staining of the islets. The diabetogenic effects of streptozotocin were completely abolished when mice were pretreated with NJE. Inhibition of streptozotocin-induced hyperglycemia by NJE was mediated by suppression of nuclear factor (NF)-kappaB activation. In addition, NJE protected against cytokine-mediated cytotoxicity. Incubation of RINm5F cells and islets with NJE resulted in a significant reduction in cytokine-induced NF-kappaB activation and downstream events, inducible nitric oxide synthase expression and nitric oxide production. The protective effect of NJE was further demonstrated by the normal insulin secretion of cytokine-treated islets in response to glucose.<br />Conclusion: NJE provided resistance to pancreatic beta-cell damage from cytokine or streptozotocin treatment. The beta-cell protective effect of NJE is mediated by suppressing NF-kappaB activation.
- Subjects :
- Animals
Base Sequence
Cell Death drug effects
Cell Line
DNA Primers genetics
In Vitro Techniques
Insulin metabolism
Insulin Secretion
Insulin-Secreting Cells pathology
Insulin-Secreting Cells physiology
Interferon-gamma antagonists & inhibitors
Interferon-gamma toxicity
Interleukin-1beta antagonists & inhibitors
Interleukin-1beta toxicity
Male
Mice
Mice, Inbred ICR
NF-kappa B metabolism
Nitric Oxide Synthase Type II genetics
Plant Extracts pharmacology
Rats
Rats, Sprague-Dawley
Signal Transduction drug effects
Cytokines antagonists & inhibitors
Cytokines toxicity
Diabetes Mellitus, Experimental prevention & control
Hypoglycemic Agents pharmacology
Insulin-Secreting Cells drug effects
Nardostachys
Subjects
Details
- Language :
- English
- ISSN :
- 2219-2840
- Volume :
- 16
- Issue :
- 26
- Database :
- MEDLINE
- Journal :
- World journal of gastroenterology
- Publication Type :
- Academic Journal
- Accession number :
- 20614480
- Full Text :
- https://doi.org/10.3748/wjg.v16.i26.3249