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The effect of incorporation of exogenous stromal cell-derived factor-1 alpha within a knitted silk-collagen sponge scaffold on tendon regeneration.
- Source :
-
Biomaterials [Biomaterials] 2010 Oct; Vol. 31 (28), pp. 7239-49. - Publication Year :
- 2010
-
Abstract
- This study developed a bioactive knitted silk-collagen sponge scaffold by incorporation of exogenous SDF-1 alpha, to enable selective migration and homing of cells for in situ tendon regeneration. With in vitro studies, it was observed that CXCR4 gene expression and migration of bone mesenchymal stromal cells and hypo-dermal fibroblasts were more sensitive to exogenous SDF-1 alpha, while expression of tendon repair gene markers by hypo-dermal fibroblasts and Achilles tendon fibroblasts were more sensitive to exogenous SDF-1 alpha. With a rat Achilles tendon injury model, exogenous SDF-1 alpha was shown to reduce infiltration of inflammatory cells and enhance migration of fibroblast-like cells into the scaffold at 4 days and 1 week post-surgery. After 4 weeks, SDF-1 alpha treated tendon had increased expression of tendon repair gene markers and endogenous SDF-1 alpha, exhibited more physiological microstructures with larger diameter collagen fibrils, and had better biomechanical properties than the control group. Hence, our bioactive scaffold improved efficacy of tendon regeneration by increasing the recruitment of fibroblast-like cells, enhancing local endogenous SDF-1 alpha and tendon extracellular matrix production, and decreasing accumulation of inflammatory cells. Incorporation of SDF-1 alpha within a knitted silk-collagen sponge scaffold can therefore be a practical application for tendon tissue engineering.
- Subjects :
- Animals
Biocompatible Materials chemistry
Biocompatible Materials metabolism
Biomarkers metabolism
Cell Movement drug effects
Cells, Cultured
Chemokine CXCL12 chemistry
Female
Fibroblasts cytology
Fibroblasts drug effects
Fibroblasts physiology
Gene Expression drug effects
Materials Testing
Rats
Regeneration physiology
Tendon Injuries drug therapy
Tendons cytology
Tissue Engineering instrumentation
Tissue Engineering methods
Chemokine CXCL12 pharmacology
Collagen chemistry
Regeneration drug effects
Silk chemistry
Tendons drug effects
Tendons physiology
Tissue Scaffolds chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1878-5905
- Volume :
- 31
- Issue :
- 28
- Database :
- MEDLINE
- Journal :
- Biomaterials
- Publication Type :
- Academic Journal
- Accession number :
- 20615544
- Full Text :
- https://doi.org/10.1016/j.biomaterials.2010.05.040