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Sal k 4, a new allergen of Salsola kali, is profilin: a predictive value of conserved conformational regions in cross-reactivity with other plant-derived profilins.
- Source :
-
Bioscience, biotechnology, and biochemistry [Biosci Biotechnol Biochem] 2010; Vol. 74 (7), pp. 1441-6. Date of Electronic Publication: 2010 Jul 07. - Publication Year :
- 2010
-
Abstract
- The aim of this study was to investigate a new allergen of Salsola kali, Sal k 4, and to investigate the predictive value of the conserved conformational regions in cross-reactivity with other plant-derived profilins. The Sal k 4-coding sequence was cloned, expressed, and purified by one-step Ni2+ affinity chromatography to recover high-purity target protein. We assessed cross-reactivity and predicted conserved conformational regions among rSal k 4 and other plant-derived profilins. Immunodetection and inhibition assays using 30 individual sera from S. kali allergic patients indicated that purified rSal k 4 might be the same as that in the crude extract. The results of inhibition assays among rSal k 4 and other plant-derived profilins were in accordance with the homology of the predicted conserved conformational regions. Amino acid sequence homology analysis showed that a high degree of IgE cross-reactivity among plant-derived profilins might depend on the predicted conserved conformational regions.
- Subjects :
- Amino Acid Sequence
Cloning, Molecular
Enzyme-Linked Immunosorbent Assay
Escherichia coli genetics
Female
Humans
Immunoglobulin E immunology
Male
Models, Molecular
Molecular Sequence Data
Plant Proteins biosynthesis
Plant Proteins chemistry
Plant Proteins immunology
Plant Proteins isolation & purification
Profilins biosynthesis
Profilins isolation & purification
Protein Conformation
Sequence Analysis, DNA
Sequence Homology, Amino Acid
Skin immunology
Allergens
Conserved Sequence
Cross Reactions
Pollen
Profilins chemistry
Profilins immunology
Salsola immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1347-6947
- Volume :
- 74
- Issue :
- 7
- Database :
- MEDLINE
- Journal :
- Bioscience, biotechnology, and biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 20622444
- Full Text :
- https://doi.org/10.1271/bbb.100129