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Analysis and stability study of temozolomide using capillary electrophoresis.
- Source :
-
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences [J Chromatogr B Analyt Technol Biomed Life Sci] 2010 Jul 01; Vol. 878 (21), pp. 1801-8. - Publication Year :
- 2010
-
Abstract
- The applicability of micellar electrokinetic capillary chromatography (MEKC) for the analysis of temozolomide (TMZ) and its degradants, 3-methyl-(triazen-1-yl)imidazole-4-carboxamide (MTIC) and 5-amino-imidazole-4-carboxamide (AIC) has been studied. Using short-end injection, the analysis of TMZ and its degradants could be performed within 1.2 min. The obtained precision of migration times was better than 1.6 RSD%, and the limit of quantitation (LOQ) was 0.31-0.93 microg/mL. The therapeutic concentration of TMZ in blood samples can be determined after direct sample injection and conventional on-capillary UV detection. The proposed MEKC method was applied to study the stability of TMZ in water and serum at different pH values. It was established that the half-life of the TMZ in vitro serum at room temperature was 33 min, close to the half-life (28 min) obtained in water at pH 7.9.
- Subjects :
- Aminoimidazole Carboxamide analysis
Aminoimidazole Carboxamide blood
Aminoimidazole Carboxamide chemistry
Antineoplastic Agents, Alkylating analysis
Antineoplastic Agents, Alkylating blood
Antineoplastic Agents, Alkylating chemistry
Dacarbazine analysis
Dacarbazine blood
Dacarbazine chemistry
Drug Stability
Half-Life
Humans
Hydrogen-Ion Concentration
Linear Models
Reproducibility of Results
Sensitivity and Specificity
Temozolomide
Temperature
Water
Chromatography, Micellar Electrokinetic Capillary methods
Dacarbazine analogs & derivatives
Subjects
Details
- Language :
- English
- ISSN :
- 1873-376X
- Volume :
- 878
- Issue :
- 21
- Database :
- MEDLINE
- Journal :
- Journal of chromatography. B, Analytical technologies in the biomedical and life sciences
- Publication Type :
- Academic Journal
- Accession number :
- 20627825
- Full Text :
- https://doi.org/10.1016/j.jchromb.2010.05.008