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DNA methylation in the rectal mucosa is associated with crypt proliferation and fecal short-chain fatty acids.
- Source :
-
Digestive diseases and sciences [Dig Dis Sci] 2011 Feb; Vol. 56 (2), pp. 387-96. Date of Electronic Publication: 2010 Jul 16. - Publication Year :
- 2011
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Abstract
- Background: DNA methylation varies throughout the normal colorectal mucosa and DNA methylation in normal appearing mucosa is associated with serrated and adenomatous neoplasia elsewhere within the colorectum.<br />Aims: The purpose of this study was to measure luminal chemistry, rectal proliferation and mucosal DNA methylation and thus determine whether regional and pathological patterns of DNA methylation could be explained by luminal and epithelial factors.<br />Methods: Twenty healthy subjects had normal rectal mucosal biopsies and a 24-h fecal collection. Rectal biopsies were analyzed for epithelial proliferation (Ki67 immunohistochemistry) and DNA methylation at 17 different markers, including "type A" markers (ESR1, GATA5, HIC1, HPP1, SFRP1), "type C" markers (MGMT, MLH1, CDKN2A, MINT1, MINT2, MINT31, IGF2, CACNA1G, NEUROG1, SOCS1, RUNX3), and LINE-1. Fecal analysis included short-chain fatty acids (SCFA), pH and ammonia. Mean "type A" and CIMP panel methylation Z-scores were calculated.<br />Results: Rectal proliferation was significantly correlated with methylation at ESR1 (ρ = 0.81, P = 0.003) and GATA5 (ρ = 0.78, P = 0.012). LINE-1 methylation was 71.7 vs. 74.1%, in patients with "low" and "high" fecal total SCFA concentration (defined by the median value), respectively (P = 0.0019). On multivariate linear regression "type A" methylation was independently associated with rectal proliferation (P = 0.001). LINE-1 methylation was directly associated with rectal proliferation (P = 0.038) and total fecal SCFA concentration (P = 0.002), and inversely associated with fecal NH(3) concentrations (P = 0.003).<br />Conclusions: DNA methylation in normal rectal mucosa is associated with crypt proliferation and fecal SCFA concentration. These associations may help to explain regional differences in DNA methylation as well as providing a possible link between the colorectal lumen and carcinogenesis.
- Subjects :
- Aberrant Crypt Foci metabolism
Aberrant Crypt Foci pathology
Adult
Aged
Ammonia analysis
Biomarkers
Colorectal Neoplasms metabolism
Colorectal Neoplasms pathology
Female
Humans
Intestinal Mucosa pathology
Male
Middle Aged
Rectum pathology
DNA Methylation physiology
Fatty Acids, Volatile analysis
Feces chemistry
Intestinal Mucosa metabolism
Rectum metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1573-2568
- Volume :
- 56
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Digestive diseases and sciences
- Publication Type :
- Academic Journal
- Accession number :
- 20635146
- Full Text :
- https://doi.org/10.1007/s10620-010-1312-4