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Probiotic Bio-Three induces Th1 and anti-inflammatory effects in PBMC and dendritic cells.
- Source :
-
World journal of gastroenterology [World J Gastroenterol] 2010 Jul 28; Vol. 16 (28), pp. 3529-40. - Publication Year :
- 2010
-
Abstract
- Aim: To investigate the immune response of peripheral blood mononuclear cells (PBMCs) and dendritic cells (DCs) that were stimulated by probiotic preparations.<br />Methods: PBMCs were isolated, cultured, and stimulated with Bio-Three (a mixture of Bacillus mesentericus, Clostridium butyricum and Enterococcus faecalis; 10(5), 10(6) and 10(7) CFU/mL for 24 h). Cytokine production of (1) circulating PBMCs; (2) PBMCs stimulated by probiotic preparation; (3) monocyte-derived DCs; and (4) DC and T cell co-culture was determined by enzyme-linked immunosorbent assay. Phenotypic analysis of circulating PBMCs was also investigated by flow cytometry. Blood was obtained from individuals who consumed Bio-Three (10(9) CFU/d B. mesentericus, C. butyricum and E. faecalis) for 2 wk, or those who did not take probiotics orally.<br />Results: In culture supernatants, interferon-gamma (IFN-gamma) and interleukin (IL)-10 production increased, but IL-4 and tumor necrosis factor-alpha (TNF-alpha) production by PBMCs decreased after 1 and 2 wk of probiotic treatment. Flow cytometry was also performed on day 14 and detected enhanced expression of CD11b, HLA-DR, CD4, CD45RA, CD25, CD44 and CD69 in response to Bio-Three. Furthermore, IL-10 and IL-12 were upregulated in supernatants of monocyte-derived DCs, and IFN-gamma and IL-10 were enhanced in supernatants of CD4(+) T cells co-cultured with DCs.<br />Conclusion: Bio-Three appeared to stimulate the Th1 immune response, downregulate pro-inflammatory cytokines (TNF-alpha) and upregulate anti-inflammatory cytokine (IL-10). Probiotics could be effective in activation of PBMCs and DCs.
- Subjects :
- Bacillus physiology
CD11b Antigen metabolism
Cells, Cultured
Clostridium butyricum physiology
Coculture Techniques
Dendritic Cells cytology
Dendritic Cells metabolism
Enterococcus faecalis physiology
HLA-DR Antigens metabolism
Humans
Interferon-gamma metabolism
Leukocytes, Mononuclear cytology
Leukocytes, Mononuclear metabolism
Lipopolysaccharide Receptors metabolism
Phenotype
T-Lymphocytes, Regulatory cytology
T-Lymphocytes, Regulatory drug effects
T-Lymphocytes, Regulatory metabolism
Th1 Cells cytology
Th1 Cells metabolism
Dendritic Cells drug effects
Interleukin-10 metabolism
Leukocytes, Mononuclear drug effects
Probiotics pharmacology
Th1 Cells drug effects
Tumor Necrosis Factor-alpha metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 2219-2840
- Volume :
- 16
- Issue :
- 28
- Database :
- MEDLINE
- Journal :
- World journal of gastroenterology
- Publication Type :
- Academic Journal
- Accession number :
- 20653061
- Full Text :
- https://doi.org/10.3748/wjg.v16.i28.3529