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Involvement of the P1 cistron in overcoming eIF4E-mediated recessive resistance against Clover yellow vein virus in pea.
- Source :
-
Molecular plant-microbe interactions : MPMI [Mol Plant Microbe Interact] 2010 Nov; Vol. 23 (11), pp. 1460-9. - Publication Year :
- 2010
-
Abstract
- Two recessive genes (cyv1 and cyv2) are known to confer resistance against Clover yellow vein virus (ClYVV) in pea. cyv2 has recently been revealed to encode eukaryotic translation initiation factor 4E (eIF4E) and is the same allele as sbm1 and wlm against other potyviruses. Although mechanical inoculation with crude sap is rarely able to cause infection of a cyv2 pea, biolistic inoculation of the infectious ClYVV cDNA clone does. At the infection foci, the breaking virus frequently emerges, resulting in systemic infection. Here, a derived cleaved-amplified polymorphic sequence analysis showed that the breakings were associated with a single nonsynonymous mutation on the ClYVV genome, corresponding to an amino-acid substitution at position 24 (isoleucine to valine) on the P1 cistron. ClYVV with the point mutation was able to break the resistance. This is a first report demonstrating that P1 is involved in eIF4E-mediated recessive resistance.
- Subjects :
- Base Sequence
Gene Expression Regulation, Plant physiology
Genome, Viral
Plant Proteins genetics
Plant Proteins metabolism
Plant Viruses pathogenicity
Point Mutation
Virulence
Virus Replication
Eukaryotic Initiation Factor-4E metabolism
Pisum sativum genetics
Pisum sativum virology
Plant Diseases genetics
Plant Diseases immunology
Plant Viruses physiology
Subjects
Details
- Language :
- English
- ISSN :
- 0894-0282
- Volume :
- 23
- Issue :
- 11
- Database :
- MEDLINE
- Journal :
- Molecular plant-microbe interactions : MPMI
- Publication Type :
- Academic Journal
- Accession number :
- 20653413
- Full Text :
- https://doi.org/10.1094/MPMI-11-09-0277