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Role of interleukin-6 in elastase-induced lung inflammatory changes in mice.
- Source :
-
Experimental lung research [Exp Lung Res] 2010 Aug; Vol. 36 (6), pp. 362-72. - Publication Year :
- 2010
-
Abstract
- Interleukin-6 (IL-6) is known to be involved in the pathogenesis of various inflammatory diseases, but its role in the development of pulmonary emphysema remains unclear. Wild-type (WT) and IL-6-deficient mice received either phosphate-buffered saline (PBS) or porcine pancreatic elastase (PPE) intratracheally. The development of emphysema was determined by measuring the mean linear intercept (Lm). The lung specimens were also subjected to immunohistochemistry for single-stranded DNA to detect apoptotic cells. Lung mechanics and airway responsiveness to inhaled methacholine were analyzed. Bronchoalveolar lavage (BAL) fluid was subjected to evaluation of inflammatory cell accumulation and cytokine measurement. PPE treatment caused significant increases in Lm and lung compliance, which was attenuated by IL-6 deficiency. The increases in apoptotic cells in the lung were attenuated in IL-6 null mice. Airway responsiveness was not affected by PPE challenge or IL-6 deficiency. Intratracheal PPE increased the cell counts in BAL fluid throughout the observation, which was suppressed in IL-6 null mice. In BAL fluid, PPE-induced increases in the levels of macrophage inflammatory protein (MIP)-1alpha and eotaxin were mitigated by IL-6 deficiency. PPE-induced up-regulation of matrix metalloproteinase (MMP)-12 in the lung was attenuated by IL-6 deficiency. These results indicate that IL-6 may play an important role in the development of elastase-induced lung inflammatory changes.
- Subjects :
- Airway Resistance
Animals
Apoptosis
Bronchial Provocation Tests
Bronchoalveolar Lavage Fluid cytology
Bronchoalveolar Lavage Fluid immunology
Cytokines metabolism
Disease Models, Animal
Gene Expression Regulation, Enzymologic
Immunohistochemistry
Interleukin-6 deficiency
Interleukin-6 genetics
Lung enzymology
Lung pathology
Lung physiopathology
Lung Compliance
Matrix Metalloproteinase 12 genetics
Mice
Mice, Knockout
Pancreatic Elastase
Pneumonia chemically induced
Pneumonia genetics
Pneumonia pathology
Pneumonia physiopathology
Pulmonary Emphysema chemically induced
Pulmonary Emphysema genetics
Pulmonary Emphysema pathology
Pulmonary Emphysema physiopathology
Respiratory Mechanics
Time Factors
Interleukin-6 metabolism
Lung immunology
Pneumonia immunology
Pulmonary Emphysema immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1521-0499
- Volume :
- 36
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Experimental lung research
- Publication Type :
- Academic Journal
- Accession number :
- 20653471
- Full Text :
- https://doi.org/10.3109/01902141003678590