Back to Search
Start Over
Expression of angiopoietin-like 4 in human gastric cancer: ANGPTL4 promotes venous invasion.
- Source :
-
Oncology reports [Oncol Rep] 2010 Sep; Vol. 24 (3), pp. 599-606. - Publication Year :
- 2010
-
Abstract
- There is strong evidence that the angiopoietin family is involved in the regulation of tumour progression, cellular growth and differentiation. Recently, it has been reported that angiopoietin-like 4 (ANGPTL4) in cancer cell promotes the metastatic process by increasing vascular permeability. To elucidate ANGPTL4 expression and its association with clinicopathological factors and prognosis in human gastric adenocarcinomas, we examined 103 cases of surgically-resected human gastric adenocarcinoma by immunohistochemistry. Among 103 cases of adenocarcinoma, 38 cases (36.9%) showed positive staining in the cytoplasm of the carcinoma cells for ANGPTL4. Histologically, papillary and mucinous adenocarcinomas showed relatively high expression of ANGPTL4 (60 and 60%, respectively). The expression of ANGPTL4 was correlated with the depth of tumour invasion (p<0.005), lymph node metastasis (p<0.001), venous invasion (p<0.00005) and TNM stage (p<0.001) in the total carcinoma. In univariate survival analysis, ANGPTL4 expression was not associated with the overall survival. RT-PCR or Western blot analysis showed the expression of mRNA or protein of ANGPTL4 in all four surgically-resected samples and all four cell lines of human gastric adenocarcinoma. ANGPTL4 expression was correlated with several clinicopathological factors, especially venous invasion. These findings suggest that the ANGPTL4 is one of the factors involved in the progression of human gastric cancer.
- Subjects :
- Adenocarcinoma genetics
Adenocarcinoma mortality
Adenocarcinoma pathology
Adenocarcinoma surgery
Angiopoietin-Like Protein 4
Angiopoietins genetics
Biomarkers, Tumor genetics
Blotting, Western
Cell Line, Tumor
Gastrectomy
Gene Expression Regulation, Neoplastic
Humans
Immunohistochemistry
Japan
Kaplan-Meier Estimate
Lymphatic Metastasis
Neoplasm Invasiveness
Neoplasm Staging
RNA, Messenger analysis
Reverse Transcriptase Polymerase Chain Reaction
Stomach Neoplasms genetics
Stomach Neoplasms mortality
Stomach Neoplasms pathology
Stomach Neoplasms surgery
Time Factors
Treatment Outcome
Adenocarcinoma metabolism
Angiopoietins metabolism
Biomarkers, Tumor metabolism
Stomach Neoplasms metabolism
Veins pathology
Subjects
Details
- Language :
- English
- ISSN :
- 1791-2431
- Volume :
- 24
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Oncology reports
- Publication Type :
- Academic Journal
- Accession number :
- 20664963
- Full Text :
- https://doi.org/10.3892/or_00000897