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Proteomic analysis of diaminochlorotriazine (DACT) adducts in three brain regions of Wistar rats.
- Source :
-
Toxicology letters [Toxicol Lett] 2010 Nov 10; Vol. 199 (1), pp. 17-21. Date of Electronic Publication: 2010 Aug 03. - Publication Year :
- 2010
-
Abstract
- Atrazine (ATRA) is the most commonly applied herbicide in the United States and is detected frequently in drinking water at significant levels. Following oral exposure, metabolism of ATRA generates diaminochlorotriazine (DACT), an electrophilic molecule capable of forming covalent protein adducts. At high doses, both ATRA and DACT can disrupt the preovulatory luteinizing hormone (LH) surge in rats, thereby altering normal reproductive function. This research was designed to identify DACT protein adducts formed in three distinct brain regions of ATRA-exposed rats, including the preoptic area (POA), medial basal hypothalamus (MBH), and cortex (CTX). Proteins with DACT adducts were identified following 2-dimensional electrophoresis (2-DE), immunodetection, and MALDI-TOF mass spectrometry analysis. Western blots from exposed animals revealed over 30 DACT-modified spots that were absent in controls. Protein spots were matched to concurrently run 2-DE gels stained with Sypro Ruby, excised, and in-gel digested with trypsin.<br /> (Copyright © 2010 Elsevier Ireland Ltd. All rights reserved.)
- Subjects :
- Administration, Oral
Animals
Atrazine administration & dosage
Atrazine chemistry
Atrazine metabolism
Blotting, Western
Cerebral Cortex chemistry
Cerebral Cortex metabolism
Electrophoresis, Gel, Two-Dimensional
Female
Herbicides administration & dosage
Herbicides toxicity
Hypothalamus, Middle chemistry
Hypothalamus, Middle metabolism
Immunoenzyme Techniques
Peptide Mapping
Preoptic Area chemistry
Preoptic Area metabolism
Proteins chemistry
Rats
Rats, Wistar
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Atrazine analogs & derivatives
Atrazine toxicity
Cerebral Cortex drug effects
Hypothalamus, Middle drug effects
Preoptic Area drug effects
Proteins metabolism
Proteomics methods
Subjects
Details
- Language :
- English
- ISSN :
- 1879-3169
- Volume :
- 199
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Toxicology letters
- Publication Type :
- Academic Journal
- Accession number :
- 20688138
- Full Text :
- https://doi.org/10.1016/j.toxlet.2010.07.014