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Proteomic analysis of diaminochlorotriazine (DACT) adducts in three brain regions of Wistar rats.

Authors :
Dooley GP
Ashley AK
Legare ME
Handa RJ
Hanneman WH
Source :
Toxicology letters [Toxicol Lett] 2010 Nov 10; Vol. 199 (1), pp. 17-21. Date of Electronic Publication: 2010 Aug 03.
Publication Year :
2010

Abstract

Atrazine (ATRA) is the most commonly applied herbicide in the United States and is detected frequently in drinking water at significant levels. Following oral exposure, metabolism of ATRA generates diaminochlorotriazine (DACT), an electrophilic molecule capable of forming covalent protein adducts. At high doses, both ATRA and DACT can disrupt the preovulatory luteinizing hormone (LH) surge in rats, thereby altering normal reproductive function. This research was designed to identify DACT protein adducts formed in three distinct brain regions of ATRA-exposed rats, including the preoptic area (POA), medial basal hypothalamus (MBH), and cortex (CTX). Proteins with DACT adducts were identified following 2-dimensional electrophoresis (2-DE), immunodetection, and MALDI-TOF mass spectrometry analysis. Western blots from exposed animals revealed over 30 DACT-modified spots that were absent in controls. Protein spots were matched to concurrently run 2-DE gels stained with Sypro Ruby, excised, and in-gel digested with trypsin.<br /> (Copyright © 2010 Elsevier Ireland Ltd. All rights reserved.)

Details

Language :
English
ISSN :
1879-3169
Volume :
199
Issue :
1
Database :
MEDLINE
Journal :
Toxicology letters
Publication Type :
Academic Journal
Accession number :
20688138
Full Text :
https://doi.org/10.1016/j.toxlet.2010.07.014