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Rhamnolipid mediated disruption of marine Bacillus pumilus biofilms.
- Source :
-
Colloids and surfaces. B, Biointerfaces [Colloids Surf B Biointerfaces] 2010 Nov 01; Vol. 81 (1), pp. 242-8. Date of Electronic Publication: 2010 Jul 13. - Publication Year :
- 2010
-
Abstract
- Removal of detrimental biofilms from surfaces exposed in the marine environment remains a challenge. A strain of Bacillus pumilus was isolated from the surface of titanium coupons immersed in seawater in the vicinity of Madras Atomic Power Station (MAPS) on the East coast of India. The bacterium formed extensive biofilms when compared to species such as Bacillus licheniformis, Pseudomonas aeruginosa PAO1 and Pseudomonas aureofaciens. A commercially available rhamnolipid was assessed for its ability to inhibit adhesion and disrupt pre-formed B. pumilus biofilms. The planktonic growth of B. pumilus cells was inhibited by concentrations >1.6mM. We studied the effect of various concentrations (0.05-100mM) of the rhamnolipid on adhesion of B. pumilus cells to polystyrene microtitre plates, wherein the effectiveness varied from 46 to 99%. Biofilms of B. pumilus were dislodged efficiently at sub-MIC concentrations, suggesting the role of surfactant activity in removing pre-formed biofilms. Scanning electron microscopy (SEM) confirmed the removal of biofilm-matrix components and disruption of biofilms by treatment with the rhamnolipid. The results suggest the possible use of rhamnolipids as efficient anti-adhesive and biofilm-disrupting agents with potential applications in controlling biofilms on surfaces.<br /> (Copyright (c) 2010 Elsevier B.V. All rights reserved.)
- Subjects :
- Bacillus classification
Bacillus ultrastructure
Biofilms growth & development
Dose-Response Relationship, Drug
India
Marine Biology
Microbial Sensitivity Tests
Microscopy, Electron, Scanning
Phylogeny
Polystyrenes chemistry
Pseudomonas physiology
Pseudomonas ultrastructure
Pseudomonas aeruginosa physiology
Pseudomonas aeruginosa ultrastructure
Seawater microbiology
Surface Properties
Bacillus physiology
Bacterial Adhesion drug effects
Biofilms drug effects
Glycolipids pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1873-4367
- Volume :
- 81
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Colloids and surfaces. B, Biointerfaces
- Publication Type :
- Academic Journal
- Accession number :
- 20688490
- Full Text :
- https://doi.org/10.1016/j.colsurfb.2010.07.013