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Increased CD127 expression on activated FOXP3+CD4+ regulatory T cells.
- Source :
-
European journal of immunology [Eur J Immunol] 2010 Sep; Vol. 40 (9), pp. 2528-38. - Publication Year :
- 2010
-
Abstract
- Regulatory T cells (Treg) are commonly identified by CD25 (IL-2R alpha) surface expression and/or intracellular expression of the FOXP3 transcription factor. In addition, Treg are also characterized by low CD127 (IL-7R alpha) expression when compared to conventional T cells and their biology in the periphery is considered essentially independent of IL-7. We further investigated CD127 expression on Treg and we demonstrated differential CD127 expression depending on Treg subsets considered. Notably, we observed high CD127 expression on inducible costimulatory molecule (ICOS)- and CD103-expressing Treg subsets. Since these two markers reflect activation status, we addressed whether Treg activation modulated CD127 expression. We demonstrated that in contrast to conventional T cells, Treg significantly upregulated CD127 expression during in vitro and in vivo activation using adoptive transfer and contact dermatitis models. High CD127 expression on Treg was also predominantly detected ex vivo in some specific sites, notably bone marrow and skin. Importantly, higher CD127 expression on Treg correlated with higher phosphorylation of STAT5 upon IL-7 exposure. High CD127 expression on Treg also provided survival advantage upon in vitro incubation with IL-7. We thus demonstrated that low CD127 expression is not an intrinsic characteristic of Treg and we identified activated Treg as a potential target of endogenous or therapeutic IL-7.
- Subjects :
- Adoptive Transfer
Animals
CD4 Antigens biosynthesis
Cell Survival
Cells, Cultured
DNA-Binding Proteins genetics
Dermatitis, Contact pathology
Disease Models, Animal
Forkhead Transcription Factors biosynthesis
Forkhead Transcription Factors genetics
Gene Expression Profiling
Interleukin-7 immunology
Interleukin-7 Receptor alpha Subunit genetics
Interleukin-7 Receptor alpha Subunit immunology
Lymphocyte Activation immunology
Mice
Mice, Inbred C57BL
Mice, Knockout
Mice, Transgenic
STAT5 Transcription Factor genetics
STAT5 Transcription Factor metabolism
T-Lymphocyte Subsets immunology
T-Lymphocyte Subsets pathology
T-Lymphocytes, Regulatory immunology
T-Lymphocytes, Regulatory pathology
Dermatitis, Contact immunology
Interleukin-7 metabolism
Interleukin-7 Receptor alpha Subunit biosynthesis
T-Lymphocyte Subsets metabolism
T-Lymphocytes, Regulatory metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1521-4141
- Volume :
- 40
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- European journal of immunology
- Publication Type :
- Academic Journal
- Accession number :
- 20690182
- Full Text :
- https://doi.org/10.1002/eji.201040531