Back to Search
Start Over
Stem cells derived from human amniotic fluid contribute to acute kidney injury recovery.
- Source :
-
The American journal of pathology [Am J Pathol] 2010 Oct; Vol. 177 (4), pp. 2011-21. Date of Electronic Publication: 2010 Aug 19. - Publication Year :
- 2010
-
Abstract
- Stem cells isolated from human amniotic fluid are gaining attention with regard to their therapeutic potential. In this work, we investigated whether these cells contribute to tubular regeneration after experimental acute kidney injury. Cells expressing stem cell markers with multidifferentiative potential were isolated from human amniotic fluid. The regenerative potential of human amniotic fluid stem cells was compared with that of bone marrow-derived human mesenchymal stem cells. We found that the intravenous injection of 3.5 × 10(5) human amniotic fluid stem cells into nonimmune-competent mice with glycerol-induced acute kidney injury was followed by rapid normalization of renal function compared with injection of mesenchymal stem cells. Both stem cell types showed enhanced tubular cell proliferation and reduced apoptosis. Mesenchymal stem cells were more efficient in inducing proliferation than amniotic fluid-derived stem cells, which, in contrast, were more antiapoptotic. Both cell types were found to accumulate within the peritubular capillaries and the interstitium, but amniotic fluid stem cells were more persistent than mesenchymal stem cells. In vitro experiments demonstrated that the two cell types produced different cytokines and growth factors, suggesting that a combination of different mediators is involved in their biological actions. These results suggest that the amniotic fluid-derived stem cells may improve renal regeneration in acute kidney injury, but they are not more effective than mesenchymal stem cells.
- Subjects :
- Acute Kidney Injury etiology
Adult
Animals
Apoptosis
Blotting, Western
Bone Marrow metabolism
Cell Differentiation
Cell Proliferation
Cells, Cultured
Female
Flow Cytometry
Humans
Male
Mice
Mice, Inbred BALB C
Mice, SCID
RNA, Messenger genetics
Reverse Transcriptase Polymerase Chain Reaction
Rhabdomyolysis etiology
Acute Kidney Injury prevention & control
Amniotic Fluid cytology
Mesenchymal Stem Cells cytology
Rhabdomyolysis prevention & control
Stem Cell Transplantation
Stem Cells cytology
Subjects
Details
- Language :
- English
- ISSN :
- 1525-2191
- Volume :
- 177
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- The American journal of pathology
- Publication Type :
- Academic Journal
- Accession number :
- 20724594
- Full Text :
- https://doi.org/10.2353/ajpath.2010.091245