NADH oxidase activity of Bacillus subtilis nitroreductase NfrA1: insight into its biological role.

Authors :: Cortial S
Chaignon P
Iorga BI
Aymerich S
Truan G
Gueguen-Chaignon V
Meyer P
Moréra S
Ouazzani J
Source :: FEBS letters [FEBS Lett] 2010 Sep 24; Vol. 584 (18), pp. 3916-22. Date of Electronic Publication: 2010 Aug 18.
Publication Year :: 2010
Abstract: NfrA1 nitroreductase from the Gram-positive bacterium Bacillus subtilis is a member of the NAD(P)H/FMN oxidoreductase family. Here, we investigated the reactivity, the structure and kinetics of NfrA1, which could provide insight into the unclear biological role of this enzyme. We could show that NfrA1 possesses an NADH oxidase activity that leads to high concentrations of oxygen peroxide and an NAD(+) degrading activity leading to free nicotinamide. Finally, we showed that NfrA1 is able to rapidly scavenge H(2)O(2) produced during the oxidative process or added exogenously.<br /> (Copyright © 2010 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.)

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