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[Effect of NAD+ against radiation injury and its dose-effect relationship].
- Source :
-
Nan fang yi ke da xue xue bao = Journal of Southern Medical University [Nan Fang Yi Ke Da Xue Xue Bao] 2010 Aug; Vol. 30 (8), pp. 1787-9. - Publication Year :
- 2010
-
Abstract
- Objective: To explore the effect of NAD+ against radiation injury and its dose-effect relationship.<br />Methods: L02 liver cells cultured in RPMI 1640 medium containing 10% fetal calf serum were exposed to X-ray irradiation followed by immediate application of NAD+. The cellular viability was analyzed by MTT assay and the apoptotic cells were detected by TUNEL methods to observe the damages of L02 liver cells induced by X-ray exposure and analyze the dose-effect relationship of NAD+.<br />Results: The viability of L02 liver cells was decreased with increasing dose of X-ray irradiation. The most obvious growth inhibition of L02 cells occurred 24 h after the irradiation. NAD+ significantly increased the cell survival rate after irradiation, and this effect was gradually increased within the concentration range of 100-1000 microg/ml; at higher concentrations, the survival rate of the irradiated L02 cells showed no significant increase.<br />Conclusion: NAD+ provides partial protection of the liver cells against radiation injury, and the effect is positively correlated to NAD+ concentration within a certain range.
- Subjects :
- Apoptosis radiation effects
Cell Line
Cell Survival radiation effects
Dose-Response Relationship, Drug
Hepatocytes cytology
Hepatocytes drug effects
Hepatocytes radiation effects
Humans
NAD administration & dosage
Radiation Injuries prevention & control
Apoptosis drug effects
Cell Survival drug effects
NAD pharmacology
Subjects
Details
- Language :
- Chinese
- ISSN :
- 1673-4254
- Volume :
- 30
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- Nan fang yi ke da xue xue bao = Journal of Southern Medical University
- Publication Type :
- Academic Journal
- Accession number :
- 20813665