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Evaluation of local immune response to Fasciola hepatica experimental infection in the liver and hepatic lymph nodes of goats immunized with Sm14 vaccine antigen.
- Source :
-
Memorias do Instituto Oswaldo Cruz [Mem Inst Oswaldo Cruz] 2010 Aug; Vol. 105 (5), pp. 698-705. - Publication Year :
- 2010
-
Abstract
- Protection against Fasciola hepatica in goats immunized with a synthetic recombinant antigen from Schistosoma mansoni fatty acid-binding protein 14 (rSm14) was investigated by assessing worm burdens, serum levels of hepatic enzymes, faecal egg count and hepatic damage, which was evaluated using gross and microscopic morphometric observation. The nature of the local immune response was assessed by examining the distribution of CD2+, CD4+, CD8+ and γ´+ T lymphocytes along with IgG+, IL-4+ and IFN-γ+ cells in the liver and hepatic lymph nodes (HLN). The goats used consisted of group 1 (unimmunized and uninfected), group 2 [infected control - immunized with Quillaia A (Quil A)] and group 3 (immunized with rSm14 in Quil A and infected), each containing seven animals. Immunization with rSm14 in Quil A adjuvant induced a reduction in gross hepatic lesions of 56.6% (p < 0.001) and reduced hepatic and HLN infiltration of CD2+, CD4+, CD8+ and γ´+ T lymphocytes as well as IL-4+ and IFN-γ+ cells (p < 0.05). This is the first report of caprine immunization against F. hepatica using a complete rSm14 molecule derived from S. mansoni. Immunization reduced hepatic damage and local inflammatory infiltration into the liver and HLN. However, considering that Quil A is not the preferential/first choice adjuvant for Sm14 immunization, further studies will be undertaken using the monophosphoryl lipid A-based family of adjuvants during clinical trials to facilitate anti-Fasciolavaccine development.
- Subjects :
- Animals
Fascioliasis prevention & control
Fatty Acid Transport Proteins administration & dosage
Goat Diseases immunology
Goats
Helminth Proteins administration & dosage
Liver immunology
Liver parasitology
Lymph Nodes immunology
Lymph Nodes parasitology
Vaccines immunology
Antigens, Helminth immunology
Fasciola hepatica immunology
Fascioliasis immunology
Fatty Acid Transport Proteins immunology
Goat Diseases parasitology
Helminth Proteins immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1678-8060
- Volume :
- 105
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Memorias do Instituto Oswaldo Cruz
- Publication Type :
- Academic Journal
- Accession number :
- 20835620
- Full Text :
- https://doi.org/10.1590/s0074-02762010000500017