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Dendritic cell-specific antigen delivery by coronavirus vaccine vectors induces long-lasting protective antiviral and antitumor immunity.
- Source :
-
MBio [mBio] 2010 Sep 14; Vol. 1 (4). Date of Electronic Publication: 2010 Sep 14. - Publication Year :
- 2010
-
Abstract
- Efficient vaccination against infectious agents and tumors depends on specific antigen targeting to dendritic cells (DCs). We report here that biosafe coronavirus-based vaccine vectors facilitate delivery of multiple antigens and immunostimulatory cytokines to professional antigen-presenting cells in vitro and in vivo. Vaccine vectors based on heavily attenuated murine coronavirus genomes were generated to express epitopes from the lymphocytic choriomeningitis virus glycoprotein, or human Melan-A, in combination with the immunostimulatory cytokine granulocyte-macrophage colony-stimulating factor (GM-CSF). These vectors selectively targeted DCs in vitro and in vivo resulting in vector-mediated antigen expression and efficient maturation of DCs. Single application of only low vector doses elicited strong and long-lasting cytotoxic T-cell responses, providing protective antiviral and antitumor immunity. Furthermore, human DCs transduced with Melan-A-recombinant human coronavirus 229E efficiently activated tumor-specific CD8(+) T cells. Taken together, this novel vaccine platform is well suited to deliver antigens and immunostimulatory cytokines to DCs and to initiate and maintain protective immunity.
- Subjects :
- Animals
Antigens genetics
Antigens immunology
Cancer Vaccines administration & dosage
Cancer Vaccines genetics
Cell Line
Cells, Cultured
Coronavirus immunology
Dendritic Cells virology
Genetic Vectors immunology
Humans
Mice
Mice, Inbred C57BL
Neoplasms immunology
Species Specificity
Viral Vaccines administration & dosage
Viral Vaccines genetics
Virus Diseases immunology
Antigens administration & dosage
Cancer Vaccines immunology
Coronavirus genetics
Dendritic Cells immunology
Genetic Vectors genetics
Neoplasms prevention & control
Viral Vaccines immunology
Virus Diseases prevention & control
Subjects
Details
- Language :
- English
- ISSN :
- 2150-7511
- Volume :
- 1
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- MBio
- Publication Type :
- Academic Journal
- Accession number :
- 20844609
- Full Text :
- https://doi.org/10.1128/mBio.00171-10