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Attenuation of cadmium-induced testicular injury in metallothionein-III null mice.
- Source :
-
Life sciences [Life Sci] 2010 Oct 23; Vol. 87 (17-18), pp. 545-50. Date of Electronic Publication: 2010 Sep 17. - Publication Year :
- 2010
-
Abstract
- Aims: In order to evaluate the role of metallothionein (MT)-III in cadmium (Cd)-induced testicular toxicity, we examined the sensitivity of MT-III null mice to severe testicular injury caused by Cd.<br />Main Methods: Male MT-III null mice, MT-I/II null mice and wild-type mice were given a subcutaneous injection of CdCl(2) (15μmol/kg). The testis was collected from each mouse at 6, 12 and 24h after Cd administration.<br />Key Findings: Testicular hemorrhages by evaluating morphology, hemoglobin content and histological parameters in the 3 types of mice were elevated by Cd injection in a time-dependent manner. The degree of hemorrhage in Cd-injected MT-I/II null mice was similar to that in the wild-type mice. In contrast, hemorrhage in the MT-III null mice was attenuated compared with that in wild-type mice and MT-I/II null mice. Cd levels, MT-I and MT-II mRNA levels and Cd-binding molecules in the testis were similar between MT-III null mice and wild-type mice. In microarray analysis, high expression of purine-nucleoside phosphorylase 2 (Pnp2), retinal degeneration 3 (Rd3), and cadherin-like 24 (Cdh24) was revealed in the testis of MT-III null mice under normal or Cd-treated conditions.<br />Significance: MT-III null mice were found to show attenuation of Cd-induced severe testicular toxicity. These results suggest the lack of MT-III contributes to protection of testis from Cd. In addition, regulation of Pnp2, Rd3, and Cdh24 mRNA levels may involve the sensitivity of MT-III null mice to Cd.<br /> (Copyright © 2010 Elsevier Inc. All rights reserved.)
- Subjects :
- Animals
Cadmium Poisoning genetics
Male
Metallothionein 3
Mice
Mice, Knockout
Nerve Tissue Proteins genetics
Testicular Diseases chemically induced
Testicular Diseases genetics
Testis drug effects
Testis injuries
Cadmium Chloride administration & dosage
Cadmium Poisoning metabolism
Cadmium Poisoning prevention & control
Nerve Tissue Proteins deficiency
Testicular Diseases prevention & control
Subjects
Details
- Language :
- English
- ISSN :
- 1879-0631
- Volume :
- 87
- Issue :
- 17-18
- Database :
- MEDLINE
- Journal :
- Life sciences
- Publication Type :
- Academic Journal
- Accession number :
- 20851133
- Full Text :
- https://doi.org/10.1016/j.lfs.2010.09.009