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Activation of p38 mitogen-activated protein kinase is critical step for acquisition of effector function in cytokine-activated T cells, but acts as a negative regulator in T cells activated through the T-cell receptor.
- Source :
-
Immunology [Immunology] 2011 Jan; Vol. 132 (1), pp. 104-10. Date of Electronic Publication: 2010 Sep 28. - Publication Year :
- 2011
-
Abstract
- Peripheral blood CD4(+) CD45RO(+) T cells activated in vitro are able to induce expression of tumour necrosis factor-α (TNF-α) in monocytes via a contact-dependent mechanism. Activation is achieved either with interleukin-2 (IL-2)/IL-6/TNF-α over an 8-day period or cross-linking CD3 using anti-CD3 antibody for 48 hr. In this paper, we show that the p38 mitogen-activated protein kinase (MAPK) signalling pathway played different roles in the generation of effector function in these two types of activated T cells. In anti-CD3 activated T cells, p38 MAPK is a negative regulator for anti-CD3 induced cell proliferation and has no significant effect on the acquisition of either the effector function (induction of monocyte-derived TNF-α) or production of T-cell cytokines. In contrast, the p38 MAPK signalling pathway is required for the acquisition of cytokine-induced effector function and promotes cell proliferation and cytokine production.
- Subjects :
- CD4-Positive T-Lymphocytes cytology
CD4-Positive T-Lymphocytes enzymology
Cell Proliferation
Cells, Cultured
Coculture Techniques
Cytokines biosynthesis
Enzyme Activation
Humans
Leukocyte Common Antigens immunology
Signal Transduction immunology
CD4-Positive T-Lymphocytes immunology
Cytokines immunology
Lymphocyte Activation
Receptors, Antigen, T-Cell immunology
p38 Mitogen-Activated Protein Kinases metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1365-2567
- Volume :
- 132
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Immunology
- Publication Type :
- Academic Journal
- Accession number :
- 20875074
- Full Text :
- https://doi.org/10.1111/j.1365-2567.2010.03345.x