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Differentially expressed RNA from public microarray data identifies serum protein biomarkers for cross-organ transplant rejection and other conditions.

Authors :
Chen R
Sigdel TK
Li L
Kambham N
Dudley JT
Hsieh SC
Klassen RB
Chen A
Caohuu T
Morgan AA
Valantine HA
Khush KK
Sarwal MM
Butte AJ
Source :
PLoS computational biology [PLoS Comput Biol] 2010 Sep 23; Vol. 6 (9). Date of Electronic Publication: 2010 Sep 23.
Publication Year :
2010

Abstract

Serum proteins are routinely used to diagnose diseases, but are hard to find due to low sensitivity in screening the serum proteome. Public repositories of microarray data, such as the Gene Expression Omnibus (GEO), contain RNA expression profiles for more than 16,000 biological conditions, covering more than 30% of United States mortality. We hypothesized that genes coding for serum- and urine-detectable proteins, and showing differential expression of RNA in disease-damaged tissues would make ideal diagnostic protein biomarkers for those diseases. We showed that predicted protein biomarkers are significantly enriched for known diagnostic protein biomarkers in 22 diseases, with enrichment significantly higher in diseases for which at least three datasets are available. We then used this strategy to search for new biomarkers indicating acute rejection (AR) across different types of transplanted solid organs. We integrated three biopsy-based microarray studies of AR from pediatric renal, adult renal and adult cardiac transplantation and identified 45 genes upregulated in all three. From this set, we chose 10 proteins for serum ELISA assays in 39 renal transplant patients, and discovered three that were significantly higher in AR. Interestingly, all three proteins were also significantly higher during AR in the 63 cardiac transplant recipients studied. Our best marker, serum PECAM1, identified renal AR with 89% sensitivity and 75% specificity, and also showed increased expression in AR by immunohistochemistry in renal, hepatic and cardiac transplant biopsies. Our results demonstrate that integrating gene expression microarray measurements from disease samples and even publicly-available data sets can be a powerful, fast, and cost-effective strategy for the discovery of new diagnostic serum protein biomarkers.

Details

Language :
English
ISSN :
1553-7358
Volume :
6
Issue :
9
Database :
MEDLINE
Journal :
PLoS computational biology
Publication Type :
Academic Journal
Accession number :
20885780
Full Text :
https://doi.org/10.1371/journal.pcbi.1000940