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Cryopreservation of Mammalian oocyte for conservation of animal genetics.
- Source :
-
Veterinary medicine international [Vet Med Int] 2010 Sep 21; Vol. 2011. Date of Electronic Publication: 2010 Sep 21. - Publication Year :
- 2010
-
Abstract
- The preservation of the female portion of livestock genetics has become an international priority; however, in situ conservation strategies are extremely expensive. Therefore, efforts are increasingly focusing on the development of a reliable cryopreservation method for oocytes, in order to establish ova banks. Slow freezing, a common method for cryopreservation of oocytes, causes osmotic shock (solution effect) and intracellular ice crystallization leading to cell damage. Vitrification is an alternative method for cryopreservation in which cells are exposed to a higher concentration of cryoprotectants and frozen with an ultra rapid freezing velocity, resulting in an ice crystal free, solid glass-like structure. Presently, vitrification is a popular method for cryopreservation of embryos. However, vitrification of oocytes is still challenging due to their complex structure and sensitivity to chilling.
Details
- Language :
- English
- ISSN :
- 2042-0048
- Volume :
- 2011
- Database :
- MEDLINE
- Journal :
- Veterinary medicine international
- Publication Type :
- Academic Journal
- Accession number :
- 20886016
- Full Text :
- https://doi.org/10.4061/2011/146405