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Successful transfer of chemical-induced asthma by adoptive transfer of low amounts of lymphocytes in a mouse model.
- Source :
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Toxicology [Toxicology] 2011 Jan 11; Vol. 279 (1-3), pp. 85-90. Date of Electronic Publication: 2010 Oct 01. - Publication Year :
- 2011
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Abstract
- Background: We optimized an adoptive transfer protocol in our mouse model of TDI-induced asthma in order to investigate the mechanisms of this type of occupational asthma.<br />Methods: On days 1 and 8, BALB/c mice were dermally sensitized with 0.3% TDI or vehicle (acetone/olive oil), and on day 15, they were sacrificed and a cell suspension was made from auricular lymph nodes. First, 0.1 x 10⁶, 0.5 x 10⁶, 1 x 10⁶ or 5 x 10⁶ cells were injected intravenously into naïve mice and three days later these mice received an oropharyngeal challenge with 0.01% TDI or vehicle. Second, mice were challenged with 0.01% TDI 1, 3, 5 or 7 days after transferring 0.5 x 10⁶ cells. The following endpoints were measured one day after challenge: methacholine reactivity; differential cell counts in bronchoalveolar lavage (BAL) and total serum IgE.<br />Results: Naïve mice receiving 0.5 x 10⁶, 1 x 10⁶ or 5 x 10⁶ cells showed significant increases in airway reactivity one day after TDI challenge; BAL neutrophils were increased after transferring 0.5 x 10⁶ and 1 x 10⁶ cells. A TDI challenge 3 days after transferring 0.5 x 10⁶ cells gave a 3-fold increase in airway resistance and a pronounced airway inflammation, whereas challenging at other time points gave no differences.<br />Conclusion: We were able to passively sensitize naïve mice using lymph node cells from TDI-sensitized mice, resulting in an asthma-like response after an airway challenge. In comparison to other adoptive transfer protocols we used substantially lower number of cells to obtain the desired response.<br /> (Copyright © 2010 Elsevier Ireland Ltd. All rights reserved.)
- Subjects :
- Animals
Bronchoalveolar Lavage Fluid immunology
Bronchoconstrictor Agents pharmacology
Immunoglobulin E blood
Male
Methacholine Chloride pharmacology
Mice
Mice, Inbred BALB C
Neutrophils immunology
Time Factors
Adoptive Transfer methods
Asthma immunology
Disease Models, Animal
Lymphocytes immunology
Toluene 2,4-Diisocyanate toxicity
Subjects
Details
- Language :
- English
- ISSN :
- 1879-3185
- Volume :
- 279
- Issue :
- 1-3
- Database :
- MEDLINE
- Journal :
- Toxicology
- Publication Type :
- Academic Journal
- Accession number :
- 20888385
- Full Text :
- https://doi.org/10.1016/j.tox.2010.09.014