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Parasympathetic innervation maintains epithelial progenitor cells during salivary organogenesis.
- Source :
-
Science (New York, N.Y.) [Science] 2010 Sep 24; Vol. 329 (5999), pp. 1645-7. - Publication Year :
- 2010
-
Abstract
- The maintenance of a progenitor cell population as a reservoir of undifferentiated cells is required for organ development and regeneration. However, the mechanisms by which epithelial progenitor cells are maintained during organogenesis are poorly understood. We report that removal of the parasympathetic ganglion in mouse explant organ culture decreased the number and morphogenesis of keratin 5-positive epithelial progenitor cells. These effects were rescued with an acetylcholine analog. We demonstrate that acetylcholine signaling, via the muscarinic M1 receptor and epidermal growth factor receptor, increased epithelial morphogenesis and proliferation of the keratin 5-positive progenitor cells. Parasympathetic innervation maintained the epithelial progenitor cell population in an undifferentiated state, which was required for organogenesis. This mechanism for epithelial progenitor cell maintenance may be targeted for organ repair or regeneration.
- Subjects :
- Acetylcholine metabolism
Animals
Carbachol metabolism
Carbachol pharmacology
Cell Differentiation
Epithelial Cells cytology
Epithelium embryology
Epithelium innervation
ErbB Receptors metabolism
Ganglia, Parasympathetic cytology
Ganglia, Parasympathetic embryology
Heparin-binding EGF-like Growth Factor
Intercellular Signaling Peptides and Proteins metabolism
Intercellular Signaling Peptides and Proteins pharmacology
Keratin-5 analysis
Keratin-5 genetics
Male
Mice
Morphogenesis drug effects
Neurons cytology
Organ Culture Techniques
Prostate cytology
Prostate embryology
Prostate innervation
Quinazolines pharmacology
Receptor, Muscarinic M1 metabolism
Regeneration
Signal Transduction
Stem Cells cytology
Submandibular Gland cytology
Epithelial Cells physiology
Ganglia, Parasympathetic physiology
Neurons physiology
Organogenesis
Stem Cells physiology
Submandibular Gland embryology
Submandibular Gland innervation
Subjects
Details
- Language :
- English
- ISSN :
- 1095-9203
- Volume :
- 329
- Issue :
- 5999
- Database :
- MEDLINE
- Journal :
- Science (New York, N.Y.)
- Publication Type :
- Academic Journal
- Accession number :
- 20929848
- Full Text :
- https://doi.org/10.1126/science.1192046