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Induction by TNF-α of IL-6 and IL-8 in cystic fibrosis bronchial IB3-1 epithelial cells encapsulated in alginate microbeads.
- Source :
-
Journal of biomedicine & biotechnology [J Biomed Biotechnol] 2010; Vol. 2010. Date of Electronic Publication: 2010 Sep 08. - Publication Year :
- 2010
-
Abstract
- We have developed a microencapsulation procedure for the entrapment and manipulation of IB3-1 cystic fibrosis cells. The applied method is based on generation of monodisperse droplets by a vibrational nozzle. Different experimental parameters were analyzed, including frequency and amplitude of vibration, polymer pumping rate and distance between the nozzle and the gelling bath. We have found that the microencapsulation procedure does not alter the viability of the encapsulated IB3-1 cells. The encapsulated IB3-1 cells were characterized in term of secretomic profile, analyzing the culture medium by Bio-Plex strategy. The experiments demonstrated that most of the analyzed proteins, were secreted both by the free and encapsulated cells, even if in a different extent. In order to determine the biotechnological applications of this procedure, we determined whether encapsulated IB3-1 cells could be induced to pro-inflammatory responses, after treatment with TNF-α. In this experimental set-up, encapsulated and free IB3-1 cells were treated with TNF-α, thereafter the culture media from both cell populations were collected. As expected, TNF-α induced a sharp increase in the secretion of interleukins, chemokines and growth factors. Of great interest was the evidence that induction of interleukin-6 and interleukin-8 occurs also by encapsulated IB3-1 cells.
- Subjects :
- Bronchi cytology
Cell Survival drug effects
Cells, Cultured
Drug Compounding methods
Gene Expression drug effects
Glucuronic Acid chemistry
Glucuronic Acid pharmacology
Hexuronic Acids chemistry
Hexuronic Acids pharmacology
Humans
Interleukin-6 genetics
Interleukin-8 genetics
Microscopy
Particle Size
Proteome metabolism
Research Design
Alginates chemistry
Alginates pharmacology
Cystic Fibrosis
Epithelial Cells cytology
Epithelial Cells metabolism
Interleukin-6 metabolism
Interleukin-8 metabolism
Microspheres
Tumor Necrosis Factor-alpha metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1110-7251
- Volume :
- 2010
- Database :
- MEDLINE
- Journal :
- Journal of biomedicine & biotechnology
- Publication Type :
- Academic Journal
- Accession number :
- 20936184
- Full Text :
- https://doi.org/10.1155/2010/907964