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Cloning and characterization of a venom allergen-like protein gene cluster from the pinewood nematode Bursaphelenchus xylophilus.
- Source :
-
Experimental parasitology [Exp Parasitol] 2011 Feb; Vol. 127 (2), pp. 440-7. - Publication Year :
- 2011
-
Abstract
- Pinewood nematode (PWN) is the causal agent of the pine wilt disease. Previous studies have suggested that secretions from the esophageal glands of PWN play an important role in pathogenicity. A cluster of three venom allergen-like protein genes and one pseudogene, Bx-vap-1, Bx-vap-2, Bx-vap-3 and Bx-vap-P, were identified within a 3.7-kb region. Additionally, three putative modification, transport and regulatory protein genes were also detected in the same flanking region of the Bx-vap gene cluster. Genes vap-1, -2 and -3 are functional and encode three major allelic variants of PWN venom allergen-like proteins. But Bx-vap-P is an untranscribed pseudogene. Genes vap-1, -2 and -3 produce predicted products of 204, 206 and 203 amino acid residues, respectively, including the putative signal peptide sequence at the amino termini. In situ mRNA hybridization analysis showed that the transcripts of genes vap-1, -2 and -3 accumulated exclusively within the esophageal gland cells of Bursaphelenchus xylophilus.<br /> (Copyright © 2010 Elsevier Inc. All rights reserved.)
- Subjects :
- Amino Acid Sequence
Animals
Blotting, Southern
Cloning, Molecular
DNA, Helminth chemistry
Gene Expression Regulation, Developmental
Helminth Proteins chemistry
Helminth Proteins immunology
Molecular Sequence Data
Phylogeny
Pinus parasitology
Plant Diseases parasitology
RNA, Messenger analysis
Sequence Alignment
Sequence Analysis, DNA
Tylenchida chemistry
Tylenchida classification
Tylenchida pathogenicity
Helminth Proteins genetics
Multigene Family genetics
Tylenchida genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1090-2449
- Volume :
- 127
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Experimental parasitology
- Publication Type :
- Academic Journal
- Accession number :
- 20971105
- Full Text :
- https://doi.org/10.1016/j.exppara.2010.10.013