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Reactive glial cells: increased stiffness correlates with increased intermediate filament expression.
- Source :
-
FASEB journal : official publication of the Federation of American Societies for Experimental Biology [FASEB J] 2011 Feb; Vol. 25 (2), pp. 624-31. Date of Electronic Publication: 2010 Oct 25. - Publication Year :
- 2011
-
Abstract
- Increased stiffness of reactive glial cells may impede neurite growth and contribute to the poor regenerative capabilities of the mammalian central nervous system. We induced reactive gliosis in rodent retina by ischemia-reperfusion and assessed intermediate filament (IF) expression and the viscoelastic properties of dissociated single glial cells in wild-type mice, mice lacking glial fibrillary acidic protein and vimentin (GFAP(-/-)Vim(-/-)) in which glial cells are consequently devoid of IFs, and normal Long-Evans rats. In response to ischemia-reperfusion, glial cells stiffened significantly in wild-type mice and rats but were unchanged in GFAP(-/-)Vim(-/-) mice. Cell stiffness (elastic modulus) correlated with the density of IFs. These results support the hypothesis that rigid glial scars impair nerve regeneration and that IFs are important determinants of cellular viscoelasticity in reactive glia. Thus, therapeutic suppression of IF up-regulation in reactive glial cells may facilitate neuroregeneration.
- Subjects :
- Animals
Biomechanical Phenomena
Glial Fibrillary Acidic Protein
Gliosis metabolism
Gliosis pathology
Mice
Mice, Knockout
Nerve Tissue Proteins genetics
Nerve Tissue Proteins metabolism
Rats
Rats, Long-Evans
Reperfusion Injury
Vimentin genetics
Vimentin metabolism
Gene Expression Regulation physiology
Intermediate Filaments metabolism
Neuroglia cytology
Neuroglia physiology
Subjects
Details
- Language :
- English
- ISSN :
- 1530-6860
- Volume :
- 25
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- FASEB journal : official publication of the Federation of American Societies for Experimental Biology
- Publication Type :
- Academic Journal
- Accession number :
- 20974670
- Full Text :
- https://doi.org/10.1096/fj.10-163790