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Effects of 8-methylguanine on structure, stability and kinetics of formation of tetramolecular quadruplexes.
- Source :
-
Biochimie [Biochimie] 2011 Mar; Vol. 93 (3), pp. 399-408. Date of Electronic Publication: 2010 Oct 27. - Publication Year :
- 2011
-
Abstract
- Tetramolecular G-quadruplexes result from the association of four guanine-rich strands. Modification of the backbone strand or the guanine bases of the oligonucleotide may improve stability or introduce new functionalities. In this regard, the 8 position of a guanosine is particularly suitable for introduction of modifications since as it is positioned in the groove of the quadruplex structure. Modifications at this position should not interfere with structural assembly as would changes at Watson-Crick and Hoogsteen sites. In this study, we investigated the effect of an 8-methyl-2'-deoxyguanosine residue (M) on the structure and stability of tetramolecular parallel G-quadruplexes. In some cases, the presence of this residue resulted in the formation of unusual quadruplex structures containing all-syn tetrads. Furthermore, the modified nucleoside M at the 5'-end of the sequence accelerated quadruplex formation by 15-fold or more relative to the unmodified oligonucleotide, which makes this nucleobase an attractive replacement for guanine in the context of tetramolecular parallel quadruplexes.<br /> (Copyright © 2010 Elsevier Masson SAS. All rights reserved.)
- Subjects :
- Base Sequence
Biophysical Phenomena
Deoxyguanosine analogs & derivatives
Kinetics
Magnetic Resonance Spectroscopy
Models, Molecular
Oligodeoxyribonucleotides chemistry
Oligodeoxyribonucleotides genetics
Oligodeoxyribonucleotides metabolism
Temperature
G-Quadruplexes
Guanine analogs & derivatives
Subjects
Details
- Language :
- English
- ISSN :
- 1638-6183
- Volume :
- 93
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Biochimie
- Publication Type :
- Academic Journal
- Accession number :
- 21034790
- Full Text :
- https://doi.org/10.1016/j.biochi.2010.10.011