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Sequencing of DC-SIGN promoter indicates an association between promoter variation and risk of nasopharyngeal carcinoma in cantonese.
- Source :
-
BMC medical genetics [BMC Med Genet] 2010 Nov 11; Vol. 11, pp. 161. Date of Electronic Publication: 2010 Nov 11. - Publication Year :
- 2010
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Abstract
- Background: The dendritic cell-specific intercellular adhesion molecule 3 grabbing non-integrin (DC-SIGN) is an important pathogen recognition receptor of the innate immune system. DC-SIGN promoter variants play important role in the susceptibility to various infectious diseases. Nasopharyngeal carcinoma (NPC) is a malignancy that is common in southern China and whether DC-SIGN promoter variants have effects on susceptibility to NPC is still unknown. The aim of this study is to ascertain the potential involvement of DC-SIGN promoter single nucleotide polymorphisms (SNPs) in NPC susceptibility.<br />Methods: We conducted a case control study based on Cantonese population including 444 NPC patients and 464 controls matched on age and sex. The 1041 bp of DC-SIGN promoter region was directly sequenced for all samples. Sequence alignment and SNP search were inspected using DNAStar analysis programs and haplotype frequencies were estimated in Haploview V 4.0. The associations between the SNPs and the risk of NPC were analyzed using chi-square test and non-conditional logistic regression analysis with SPSS 13.0 software.<br />Results: A total of six variants were observed in the DC-SIGN promoter region and DC-SIGN -139 GG and -939 AA were significantly associated with NPC risk with adjusted Odds Ratios (ORs) of 2.10 (95% confidence interval [CI] = 1.23-3.59; P = 0.006) and 2.52 (1.29-4.93; P = 0.007) respectively and subjects carrying the risk allele DC-SIGN -871 G had 1.47-fold (95% CI = 1.14-1.90) increased risks of developing NPC (P = 0.003). Haplotype analysis revealed that h1 'AAAG' was significantly associated with protection against NPC (OR = 0.69; P = 0.0002) and the association was still significant when using 1000 permutation test runs (P = 0.001).<br />Conclusions: Our study indicated that DC-SIGN promoter variants appear to be involved in the susceptibility to NPC and the detailed mechanism of this effect need further studies.
- Subjects :
- Adult
Carcinoma
Case-Control Studies
China epidemiology
Female
Genetic Predisposition to Disease
Haplotypes
Humans
Male
Middle Aged
Nasopharyngeal Carcinoma
Nasopharyngeal Neoplasms epidemiology
Nasopharyngeal Neoplasms genetics
Polymorphism, Single Nucleotide
Risk
Cell Adhesion Molecules genetics
Genetic Association Studies
Genetic Variation
Lectins, C-Type genetics
Promoter Regions, Genetic
Receptors, Cell Surface genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1471-2350
- Volume :
- 11
- Database :
- MEDLINE
- Journal :
- BMC medical genetics
- Publication Type :
- Academic Journal
- Accession number :
- 21067616
- Full Text :
- https://doi.org/10.1186/1471-2350-11-161