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The genes for three xylan-degrading activities from Bacteroides ovatus are clustered in a 3.8-kilobase region.
- Source :
-
Journal of bacteriology [J Bacteriol] 1990 May; Vol. 172 (5), pp. 2408-12. - Publication Year :
- 1990
-
Abstract
- Genes coding for three xylan-degrading activities, xylanase, xylosidase, and arabinosidase, were simultaneously cloned from the colonic anaerobic organism Bacteriodes ovatus. The genes for the three enzymes were located on a 3.8-kilobase EcoRI genomic insert and were cloned by using plasmid pUC18. All three activities were expressed in Escherichia coli JM83, and all were cell associated. Expression of the xylanase gene was independent from expression of the xylosidase and arabinosidase genes, whereas expression of the latter two genes appeared to be coordinated. Restriction endonuclease analysis of the arabinosidase and xylosidase genes and partial purification of these enzyme activities from E. coli suggested that these activities were catalyzed by a bifunctional protein or two proteins of very similar molecular weight. All three enzyme activities were regulated in B. ovatus in response to the carbon source used for growth. This is the first report of the cloning and expression of B. ovatus genes.
- Subjects :
- Bacteroides enzymology
Chromatography, Thin Layer
Cloning, Molecular
Electrophoresis, Polyacrylamide Gel
Enzyme Induction
Escherichia coli genetics
Glycoside Hydrolases biosynthesis
Glycoside Hydrolases metabolism
Molecular Weight
Oligosaccharides isolation & purification
Plasmids
Restriction Mapping
Substrate Specificity
Xylan Endo-1,3-beta-Xylosidase
Xylosidases biosynthesis
Xylosidases metabolism
Bacteroides genetics
Genes, Bacterial
Glycoside Hydrolases genetics
Multigene Family
Polysaccharides metabolism
Xylans metabolism
Xylosidases genetics
Subjects
Details
- Language :
- English
- ISSN :
- 0021-9193
- Volume :
- 172
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Journal of bacteriology
- Publication Type :
- Academic Journal
- Accession number :
- 2110141
- Full Text :
- https://doi.org/10.1128/jb.172.5.2408-2412.1990