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Alkynyl-farnesol reporters for detection of protein S-prenylation in cells.
- Source :
-
Molecular bioSystems [Mol Biosyst] 2011 Jan; Vol. 7 (1), pp. 67-73. Date of Electronic Publication: 2010 Nov 25. - Publication Year :
- 2011
-
Abstract
- Protein S-prenylation is a lipid modification that regulates membrane-protein and protein-protein interactions in cell signaling. Though sites of protein S-prenylation can be predicted based upon conserved C-terminal CaaX or CC/CXC motifs, biochemical detection of protein S-prenylation in cells is still challenging. Herein, we report an alkynyl-isoprenol chemical reporter (alk-FOH) as an efficient substrate for prenyltransferases in mammalian cells that enables sensitive detection of S-farnesylated and S-geranylgeranylated proteins using bioorthogonal ligation methods. Fluorescent detection alleviates the need to deplete cellular isoprenoids for biochemical analysis of S-prenylated proteins and enables robust characterization of S-prenylated proteins, such as effectors that are injected into host cells by bacterial pathogens. This alkynyl-prenylation reporter provides a sensitive tool for biochemical analysis and rapid profiling of prenylated proteins in cells.
Details
- Language :
- English
- ISSN :
- 1742-2051
- Volume :
- 7
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Molecular bioSystems
- Publication Type :
- Academic Journal
- Accession number :
- 21107478
- Full Text :
- https://doi.org/10.1039/c0mb00183j