Development of rHA1-ELISA for specific and sensitive detection of H9 subtype influenza virus.

Authors :: Shahsavandi S
Salmanian AH
Ghorashi SA
Masoudi S
Fotouhi F
Ebrahimi MM
Source :: Journal of virological methods [J Virol Methods] 2011 Jan; Vol. 171 (1), pp. 260-3. Date of Electronic Publication: 2010 Nov 24.
Publication Year :: 2011
Abstract: A recombinant antigen-based single serum dilution ELISA was developed for simultaneous detection and subtyping of influenza viruses. Recombinant baculovirus encoding the hemagglutinin (HA(1) subunit) of H9N2 virus was generated. To evaluate the rHA1-ELISA, microplates were coated with purified HA1 protein and tested with reference control sera. Subsequently, 92 field sera collected from chickens suspected to be infected with H9N2 AIV were employed to test the efficacy of the rHA1-ELISA. The sera were tested simultaneously by HI and a commercial AIV ELISA kit. The rHA1-ELISA appeared to be highly specific and sensitive for direct detection of H9N2 antibodies in serum samples.<br /> (Copyright © 2010 Elsevier B.V. All rights reserved.)

Subjects :: Animals
Chickens
Enzyme-Linked Immunosorbent Assay methods
Influenza in Birds virology
Recombinant Proteins genetics
Sensitivity and Specificity
Antibodies, Viral blood
Hemagglutinins, Viral genetics
Influenza A Virus, H9N2 Subtype immunology
Influenza A Virus, H9N2 Subtype isolation & purification
Influenza in Birds diagnosis
Virology methods

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