Back to Search
Start Over
Detection of circulating tumor cells is improved by drug-induced antigen up-regulation: preclinical and clinical studies.
- Source :
-
Anticancer research [Anticancer Res] 2010 Nov; Vol. 30 (11), pp. 4721-30. - Publication Year :
- 2010
-
Abstract
- (51)Cr-prelabelled colon cancer cells (simulating 'circulating tumor cells', CTCs) were added to human peripheral blood and exposed to staurosporine (ST) to increase carcinoembryonic antigen (CEA) expression. CTCs were captured with immunomagnetic beads coated with Ber-EP4 monoclonal antibody, recognizing the common epithelial antigen present in the majority of cancer cells of epithelial origin, with capture efficiency of more than 80%. Moreover, ST treatment increased CEA expression without compromising Ber-EP4 capture efficiency. In a pilot clinical study on 37 patients, CTCs were captured using Ber-EP4 beads, and recognized by RT-PCR set for CEA or cytokeratin-19 (CK) mRNA detection. The results showed that: (a) the percentage of CEA-positive CTCs (CTC(CEA), 54.1%) was lower than that of CK-positive CTCs (CTC(CK), 70.3%); (b) in vitro ST treatment converted a significant number of CTC(CEA)-negative into CTC(CEA)-positive cases. Therefore, immunomagnetic capture combined with exposure to ST provides a feasible and sensitive technique for the detection of functionally-active CTCs responsive to ST-mediated CEA up-regulation.
- Subjects :
- Aged
Aged, 80 and over
Animals
Antibodies, Monoclonal immunology
Antibodies, Monoclonal metabolism
Antimetabolites, Antineoplastic pharmacology
Carcinoembryonic Antigen metabolism
Cell Line, Tumor
Colonic Neoplasms immunology
Enzyme Inhibitors pharmacology
Female
Fluorouracil pharmacology
Humans
Immunoblotting
Immunomagnetic Separation
Keratin-19 metabolism
Male
Mice
Middle Aged
Neoplasm Metastasis
Neoplastic Cells, Circulating drug effects
Neoplastic Cells, Circulating metabolism
RNA, Messenger genetics
Reverse Transcriptase Polymerase Chain Reaction
Up-Regulation
Biomarkers, Tumor analysis
Carcinoembryonic Antigen genetics
Colonic Neoplasms blood
Keratin-19 genetics
Neoplastic Cells, Circulating pathology
Staurosporine pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1791-7530
- Volume :
- 30
- Issue :
- 11
- Database :
- MEDLINE
- Journal :
- Anticancer research
- Publication Type :
- Academic Journal
- Accession number :
- 21115931