The β-adrenoceptor agonist isoproterenol promotes the activity of respiratory chain complex I and lowers cellular reactive oxygen species in fibroblasts and heart myoblasts.

Authors :: De Rasmo D
Gattoni G
Papa F
Santeramo A
Pacelli C
Cocco T
Micelli L
Sardaro N
Larizza M
Scivetti M
Milano S
Signorile A
Source :: European journal of pharmacology [Eur J Pharmacol] 2011 Feb 10; Vol. 652 (1-3), pp. 15-22. Date of Electronic Publication: 2010 Nov 29.
Publication Year :: 2011
Abstract: A study is presented on the effect of the β-adrenoceptor agonist isoproterenol on mitochondrial oxygen metabolism in fibroblast and heart myoblast cultures. Isoproterenol treatment of serum-limited fibroblasts and proliferating myoblasts results in the promotion of mitochondrial complex I activity and decrease of the cellular level of reactive oxygen species. These effects of isoproterenol are associated with cAMP-dependent phosphorylation of complex I subunit(s). Addition of okadaic acid, inhibitor of protein phosphatase(s), reverses the decline of complex I activity in serum-limited fibroblast cultures and activates the complex in proliferating myoblast cultures. The effects of isoproterenol on complex I activity and reactive oxygen species balance can contribute to the therapeutic effect of the drug.<br /> (2010 Elsevier B.V. All rights reserved.)

Subjects :: Cells, Cultured
Cyclic AMP metabolism
Fibroblasts cytology
Fibroblasts metabolism
Humans
Infant, Newborn
Mitochondria metabolism
Myoblasts, Cardiac cytology
Myoblasts, Cardiac metabolism
Phosphorylation
Adrenergic beta-Agonists pharmacology
Electron Transport Complex I metabolism
Fibroblasts drug effects
Isoproterenol pharmacology
Myoblasts, Cardiac drug effects
Reactive Oxygen Species metabolism

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