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Integration of light- and brassinosteroid-signaling pathways by a GATA transcription factor in Arabidopsis.
- Source :
-
Developmental cell [Dev Cell] 2010 Dec 14; Vol. 19 (6), pp. 872-83. - Publication Year :
- 2010
-
Abstract
- Light and brassinosteroid (BR) antagonistically regulate the developmental switch from etiolation in the dark to photomorphogenesis in the light in plants. Here, we identify GATA2 as a key transcriptional regulator that mediates the crosstalk between BR- and light-signaling pathways. Overexpression of GATA2 causes constitutive photomorphogenesis in the dark, whereas suppression of GATA2 reduces photomorphogenesis caused by light, BR deficiency, or the constitutive photomorphogenesis mutant cop1. Genome profiling and chromatin immunoprecipitation experiments show that GATA2 directly regulates genes that respond to both light and BR. BR represses GATA2 transcription through the BR-activated transcription factor BZR1, whereas light causes accumulation of GATA2 protein and feedback inhibition of GATA2 transcription. Dark-induced proteasomal degradation of GATA2 is dependent on the COP1 E3 ubiquitin ligase, and COP1 can ubiquitinate GATA2 in vitro. This study illustrates a molecular framework for antagonistic regulation of gene expression and seedling photomorphogenesis by BR and light.<br /> (Copyright © 2010 Elsevier Inc. All rights reserved.)
- Subjects :
- Arabidopsis genetics
Arabidopsis radiation effects
Base Sequence
Binding Sites genetics
DNA Primers genetics
DNA-Binding Proteins
GATA2 Transcription Factor genetics
Gene Expression Regulation, Plant
Genes, Plant
Light
MicroRNAs genetics
Models, Biological
Mutation
Phototrophic Processes genetics
Phototrophic Processes physiology
Phototrophic Processes radiation effects
Plant Growth Regulators metabolism
Plants, Genetically Modified
Promoter Regions, Genetic
RNA, Plant genetics
Signal Transduction
Steroids, Heterocyclic metabolism
Ubiquitin-Protein Ligases metabolism
Arabidopsis metabolism
Arabidopsis Proteins metabolism
GATA2 Transcription Factor metabolism
Nuclear Proteins metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1878-1551
- Volume :
- 19
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Developmental cell
- Publication Type :
- Academic Journal
- Accession number :
- 21145502
- Full Text :
- https://doi.org/10.1016/j.devcel.2010.10.023