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A phospholipase A₂ from Bothrops asper snake venom activates neutrophils in culture: expression of cyclooxygenase-2 and PGE₂ biosynthesis.
- Source :
-
Toxicon : official journal of the International Society on Toxinology [Toxicon] 2011 Feb; Vol. 57 (2), pp. 288-96. Date of Electronic Publication: 2010 Dec 10. - Publication Year :
- 2011
-
Abstract
- In this study, the production of prostaglandin E₂ (PGE₂) and up-regulation in cyclooxygenase (COX) pathway induced by a phospholipase A₂ (PLA₂), myotoxin-III (MT-III), purified from Bothrops asper snake venom, in isolated neutrophils were investigated. The arachidonic acid (AA) production and the participation of intracellular PLA₂s (cytosolic PLA₂ and Ca(2+)-independent PLA₂) in these events were also evaluated. MT-III induced COX-2, but not COX-1 gene and protein expression in neutrophils and increased PGE₂ levels. Pretreatment of neutrophils with COX-2 and COX-1 inhibitors reduced PGE₂ production induced by MT-III. Arachidonyl trifluoromethyl ketone (AACOCF₃), an intracellular PLA₂ inhibitor, but not bromoenol lactone (BEL), an iPLA₂ inhibitor, suppressed the MT-III-induced AA and PGE₂ release. In conclusion, MT-III directly stimulates neutrophils inducing COX-2 mRNA and protein expression followed by production of PGE₂. COX-2 isoform is preeminent over COX-1 for production of PGE₂ stimulated by MT-III. PGE₂ and AA release by MT-III probably is related to cPLA₂ activation.<br /> (Copyright © 2010 Elsevier Ltd. All rights reserved.)
- Subjects :
- Animals
Arachidonic Acid metabolism
Cyclooxygenase Inhibitors pharmacology
Male
Mice
Up-Regulation
Bothrops
Crotalid Venoms chemistry
Cyclooxygenase 2 metabolism
Dinoprostone metabolism
Group II Phospholipases A2 pharmacology
Neutrophil Activation drug effects
Neutrophils drug effects
Phospholipases A2 pharmacology
Reptilian Proteins pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1879-3150
- Volume :
- 57
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Toxicon : official journal of the International Society on Toxinology
- Publication Type :
- Academic Journal
- Accession number :
- 21147147
- Full Text :
- https://doi.org/10.1016/j.toxicon.2010.12.004