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Yeast response and tolerance to polyamine toxicity involving the drug : H+ antiporter Qdr3 and the transcription factors Yap1 and Gcn4.
- Source :
-
Microbiology (Reading, England) [Microbiology (Reading)] 2011 Apr; Vol. 157 (Pt 4), pp. 945-956. Date of Electronic Publication: 2010 Dec 09. - Publication Year :
- 2011
-
Abstract
- The yeast QDR3 gene encodes a plasma membrane drug : H(+) antiporter of the DHA1 family that was described as conferring resistance against the drugs quinidine, cisplatin and bleomycin and the herbicide barban, similar to its close homologue QDR2. In this work, a new physiological role for Qdr3 in polyamine homeostasis is proposed. QDR3 is shown to confer resistance to the polyamines spermine and spermidine, but, unlike Qdr2, also a determinant of resistance to polyamines, Qdr3 has no apparent role in K(+) homeostasis. QDR3 transcription is upregulated in yeast cells exposed to spermine or spermidine dependent on the transcription factors Gcn4, which controls amino acid homeostasis, and Yap1, the main regulator of oxidative stress response. Yap1 was found to be a major determinant of polyamine stress resistance in yeast and is accumulated in the nucleus of yeast cells exposed to spermidine-induced stress. QDR3 transcript levels were also found to increase under nitrogen or amino acid limitation; this regulation is also dependent on Gcn4. Consistent with the concept that Qdr3 plays a role in polyamine homeostasis, QDR3 expression was found to decrease the intracellular accumulation of [(3)H]spermidine, playing a role in the maintenance of the plasma membrane potential in spermidine-stressed cells.
- Subjects :
- Amino Acids metabolism
Nitrogen metabolism
Polyamines metabolism
Potassium metabolism
Saccharomyces cerevisiae metabolism
Saccharomyces cerevisiae physiology
Transcription, Genetic
Basic-Leucine Zipper Transcription Factors metabolism
Gene Expression Regulation, Fungal
Membrane Transport Proteins metabolism
Polyamines toxicity
Saccharomyces cerevisiae drug effects
Saccharomyces cerevisiae Proteins metabolism
Stress, Physiological
Transcription Factors metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1465-2080
- Volume :
- 157
- Issue :
- Pt 4
- Database :
- MEDLINE
- Journal :
- Microbiology (Reading, England)
- Publication Type :
- Academic Journal
- Accession number :
- 21148207
- Full Text :
- https://doi.org/10.1099/mic.0.043661-0