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Xanthine oxidase inhibitory triterpenoid and phloroglucinol from guttiferaceous plants inhibit growth and induced apoptosis in human NTUB1 cells through a ROS-dependent mechanism.
- Source :
-
Journal of agricultural and food chemistry [J Agric Food Chem] 2011 Jan 12; Vol. 59 (1), pp. 407-14. Date of Electronic Publication: 2010 Dec 15. - Publication Year :
- 2011
-
Abstract
- A known triterpenoid, β-amyrin (1), and a known and a new phloroglucinol, cohulupone (2) and garcinielliptone P (3), were isolated from the pericarp and heartwood and seed of Garcinia subelliptica, respectively. A new xanthonolignoid, hyperielliptone HF (4), was isolated from the heartwood of Hypericum geminiflorum. The new compounds were established by analysis of their spectroscopic data. Compounds 1-3 showed an inhibitory effect on xanthine oxidase (XO). Treatment of NTUB1, a human bladder cancer cell, with 1 or 1 cotreated with cisplatin for 24 h resulted in a decreased viability of cells. Exposure of NTUB1 to 1 or 1 cotreated with cisplatin for 24 h significantly increased the level of production of reactive oxygen species (ROS). Flow cytometric analysis indicated that treatment of NTUB1 with 1 or 1 cotreated with cisplatin led to the cell cycle arrest, accompanied by an increase in the extent of apoptotic cell death in 1 or 1 combined with cisplatin-treated NTUB1 after 24 h. These data suggested that the presentation of cell cycle arrest and apoptosis in 1 or 1 combined with cisplatin-treated NTUB1 for 24 h was mediated through an increased amount of ROS in cells exposed to 1 or 1 cotreated with cisplatin.
- Subjects :
- Cell Cycle drug effects
Cell Line, Tumor
Cisplatin pharmacology
Down-Regulation
Humans
Oleanolic Acid pharmacology
Urinary Bladder Neoplasms drug therapy
Urinary Bladder Neoplasms metabolism
Urinary Bladder Neoplasms physiopathology
Xanthine Oxidase antagonists & inhibitors
Apoptosis drug effects
Cell Proliferation drug effects
Enzyme Inhibitors pharmacology
Garcinia chemistry
Oleanolic Acid analogs & derivatives
Phloroglucinol pharmacology
Plant Extracts pharmacology
Reactive Oxygen Species metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1520-5118
- Volume :
- 59
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Journal of agricultural and food chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 21158429
- Full Text :
- https://doi.org/10.1021/jf1041382