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Florfenicol inhibits allergic airway inflammation in mice by p38 MAPK-mediated phosphorylation of GATA 3.
- Source :
-
Clinical immunology (Orlando, Fla.) [Clin Immunol] 2011 Feb; Vol. 138 (2), pp. 231-8. Date of Electronic Publication: 2010 Dec 15. - Publication Year :
- 2011
-
Abstract
- Florfenicol has been shown to possess anti-inflammatory activity. However, its possible use for asthma has not yet been studied. First we investigated the anti-inflammatory properties of florfenicol using mice asthma model. BALB/c mice were immunized and challenged by ovalbumin. Treatment with florfenicol caused a marked reduction in inflammatory cells and three Th2 type cytokines in the bronchoalveolar lavage fluids of mice. The levels of ovalbumin-specific IgE and airway hyperresponsiveness were significantly altered after treatment with florfenicol. Histological studies using H&E and AB-PAS staining demonstrate that florfenicol substantially inhibited ovalbumin-induced inflammatory cells infiltration in lung tissue and goblet cell hyperplasia in the airway. These results were similar to those obtained with dexamethasone treatment. We then investigated which signal transduction mechanisms could be implicated in florfenicol activity. Our results suggested that the protective effect of florfenicol was mediated by the inhibition of the p38 MAPK-mediated phosphorylation of GATA 3.<br /> (Copyright © 2010 Elsevier Inc. All rights reserved.)
- Subjects :
- Active Transport, Cell Nucleus drug effects
Animals
Asthma enzymology
Bronchoalveolar Lavage Fluid cytology
Cytokines analysis
Dexamethasone pharmacology
Disease Models, Animal
Eosinophils drug effects
Female
Goblet Cells drug effects
Immunoglobulin E analysis
Lung drug effects
Macrophages drug effects
Mice
Mice, Inbred BALB C
Neutrophils drug effects
Phosphorylation
Th2 Cells drug effects
Thiamphenicol therapeutic use
Anti-Bacterial Agents therapeutic use
Anti-Inflammatory Agents, Non-Steroidal therapeutic use
Asthma drug therapy
GATA3 Transcription Factor metabolism
Thiamphenicol analogs & derivatives
p38 Mitogen-Activated Protein Kinases metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1521-7035
- Volume :
- 138
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Clinical immunology (Orlando, Fla.)
- Publication Type :
- Academic Journal
- Accession number :
- 21163707
- Full Text :
- https://doi.org/10.1016/j.clim.2010.11.008