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A combination of methods to evaluate biofilm production may help to determine the clinical relevance of Staphylococcus in blood cultures.

A combination of methods to evaluate biofilm production may help to determine the clinical relevance of Staphylococcus in blood cultures.

Authors :
Iorio NL
Lopes AP
Schuenck RP
Barcellos AG
Olendzki AN
Lopez GL
dos Santos KR
Source :
Microbiology and immunology [Microbiol Immunol] 2011 Jan; Vol. 55 (1), pp. 28-33.
Publication Year :
2011

Abstract

Staphylococcus is the most prevalent pathogen causing bacteremia and many of its isolates possess the ability to form biofilm. In this study Staphylococcus isolates from the blood of patients with bacteremia were analyzed by two biofilm detection phenotypic methods: Congo red agar (CRA) and microtiter-plate adherence (MPA) in relation to the presence of ica genes, detected by PCR. Their oxacillin susceptibility was also evaluated. Among 127 isolates evaluated, 47 were S. aureus and 80 were coagulase negative staphylococci (CNS). Seventy-four (58.3%) isolates were mecA gene positive (27.7%S. aureus and 76.3% CNS isolates). Among the 40 S. aureus isolates which were positive for the ica genes, 25 (62.5%) were positive in MPA and 27 (67.5%) in CRA, whereas both methods combined detected 34 (85%) isolates as biofilm producers. Among 12 S. epidermidis isolates carrying ica genes, 8 were positive in MPA and 5 in CRA. The combination of CRA and MPA methods provided a better prediction of the presence of ica genes in S. aureus isolates than did either method alone.<br /> (© 2010 The Societies and Blackwell Publishing Asia Pty Ltd.)

Details

Language :
English
ISSN :
0385-5600
Volume :
55
Issue :
1
Database :
MEDLINE
Journal :
Microbiology and immunology
Publication Type :
Academic Journal
Accession number :
21175771
Full Text :
https://doi.org/10.1111/j.1348-0421.2010.00288.x