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Nucleic acids determination using the complex of eriochrome black T and silver nanoparticles in a resonance light scattering technique.
- Source :
-
Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy [Spectrochim Acta A Mol Biomol Spectrosc] 2011 Feb; Vol. 78 (2), pp. 681-6. Date of Electronic Publication: 2010 Dec 07. - Publication Year :
- 2011
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Abstract
- A novel method for the determination of nucleic acids by using silver nanoparticle (AgNPs)-eriochrome black T (EBT) as a resonance light scattering (RLS) probe has been developed. Under optimum conditions, there are linear relationships between the quenching extent of RLS intensity and the concentration of nucleic acids in the range of 4.0×10(-9)-4.0×10(-7), 4.0×10(-7)-1.6×10(-6) g mL(-1) for fish sperm DNA (fsDNA) and 4.0×10(-8)-2.0×10(-6) g mL(-1) for yeast RNA (yRNA). Their detection limits (S/N=3) are 2.0 ng mL(-1) and 21 ng mL(-1), respectively. The results indicate that AgNPs can form wirelike aggregates and nanoslices in the presence of the EBT. Whereas, when nucleic acids are added into the AgNPs-EBT system, the dynamic balance of AgNPs-EBT system is destroyed and the nanoparticles undergo dispersion again, leading to the RLS intensity of AgNPs-EBT system quenching. Meanwhile, the conformation of fsDNA is changed by the synergistic effect of AgNPs and EBT.<br /> (Copyright © 2010 Elsevier B.V. All rights reserved.)
- Subjects :
- Absorption
Animals
Calibration
Cattle
Circular Dichroism
DNA ultrastructure
Fishes
Hydrogen-Ion Concentration
Limit of Detection
Male
Metal Nanoparticles ultrastructure
Spermatozoa
Azo Compounds chemistry
Light
Metal Nanoparticles chemistry
Nucleic Acids analysis
Scattering, Radiation
Silver chemistry
Spectrum Analysis methods
Subjects
Details
- Language :
- English
- ISSN :
- 1873-3557
- Volume :
- 78
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy
- Publication Type :
- Academic Journal
- Accession number :
- 21183399
- Full Text :
- https://doi.org/10.1016/j.saa.2010.11.048