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Angiogenic activity of an enamel matrix derivative (EMD) and EMD-derived proteins: an experimental study in mice.
- Source :
-
Journal of clinical periodontology [J Clin Periodontol] 2011 Mar; Vol. 38 (3), pp. 253-60. Date of Electronic Publication: 2010 Dec 27. - Publication Year :
- 2011
-
Abstract
- Objectives: To determine whether all or only certain proteins in an enamel matrix derivative (EMD) are angiogenic.<br />Materials and Methods: The angiogenic effect was analysed using an in vivo angiogenesis assay. Silicon tubes were filled with or without potential and known angiogenic-modulating factors: (i) an EMD parent, (ii) nine pools of EMD proteins, (iii) fibroblast growth factor/vascular endothelial growth factor and (iv) amelogenin. Silicon tubes were implanted subcutaneously in mice. Dextran-fluorescein isothiocyanate (FITC) was injected via the tail vein, mice were euthanized and tubes were retrieved. Neovascularization was determined by measuring the amount of dextran-FITC within the tubes.<br />Results: The greatest angiogenic potential of the EMD parent was at a weight of 125 ng, resulting in a 4.3-fold increase compared with the negative control. Five pools of EMD proteins showed a stronger angiogenic activity than the EMD parent. Pool 5 showed the greatest angiogenic activity, when compared with the negative control (8.1-fold increase) and with 125 ng of the EMD parent (4.2-fold increase). Amelogenin demonstrated a significantly higher angiogenic activity than the negative control (increase up to 4.0-fold) and the EMD parent (increase up to 1.6-fold).<br />Conclusions: EMD parent, recombinant porcine amelogenin and certain pools of EMD proteins induced significant angiogenesis compared with the controls using a standardized in vivo assay.<br /> (© 2010 John Wiley & Sons A/S.)
- Subjects :
- Amelogenin pharmacology
Animals
Dextrans
Diffusion Chambers, Culture
Dose-Response Relationship, Drug
Endothelial Cells drug effects
Endothelial Cells pathology
Endothelium, Vascular drug effects
Endothelium, Vascular pathology
Female
Fibroblast Growth Factors pharmacology
Fluorescein-5-isothiocyanate analogs & derivatives
Fluorescent Dyes
Mice
Mice, Nude
Models, Animal
Neovascularization, Physiologic drug effects
Serum Albumin, Bovine pharmacology
Subcutaneous Tissue blood supply
Vascular Endothelial Growth Factor A pharmacology
Angiogenesis Inducing Agents pharmacology
Dental Enamel Proteins pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1600-051X
- Volume :
- 38
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Journal of clinical periodontology
- Publication Type :
- Academic Journal
- Accession number :
- 21198764
- Full Text :
- https://doi.org/10.1111/j.1600-051X.2010.01656.x